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dc.creatorPla, Luises
dc.creatorAviñó, Annaes
dc.creatorEritja, Ramónes
dc.creatorRuiz-Gaitán, Albaes
dc.creatorPemán, Javieres
dc.creatorFriaza, Vicentees
dc.creatorCalderón Sandubete, Enrique Josées
dc.creatorSantiago-Felipe, Saraes
dc.date.accessioned2024-01-08T08:53:48Z
dc.date.available2024-01-08T08:53:48Z
dc.date.issued2020
dc.identifier.citationPla, L., Aviñó, A., Eritja, R., Ruiz-Gaitán, A., Pemán, J., Friaza, V.,...,Santiago-Felipe, S. (2020). Triplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Samples. Journal of Fungi, 6 (4), 1-14. https://doi.org/10.3390/jof6040292.
dc.identifier.issn2309-608Xes
dc.identifier.urihttps://hdl.handle.net/11441/153002
dc.description.abstractPneumocystis pneumonia (PcP) is a disease produced by the opportunistic infection of the fungus Pneumocystis jirovecii. As delayed or unsuitable treatments increase the risk of mortality, the development of rapid and accurate diagnostic tools for PcP are of great importance. Unfortunately, current standard methods present severe limitations and are far from adequate. In this work, a time-competitive, sensitive and selective biosensor based on DNA-gated nanomaterials for the identification of P. jirovecii is presented. The biosensor consists of a nanoporous anodic alumina (NAA) scaffold which pores are filled with a dye reporter and capped with specific DNA oligonucleotides. In the presence of P. jirovecii genomic DNA, the gated biosensor is open, and the cargo is delivered to the solution where it is monitored through fluorescence spectroscopy. The use of capping oligonucleotides able to form duplex or triplex with P. jirovecii DNA is studied. The final diagnostic tool shows a limit of detection (LOD) of 1 nM of target complementary DNA and does not require previous amplification steps. The method was applied to identify DNA from P. jirovecii in unmodified bronchoalveolar lavage, nasopharyngeal aspirates, and sputum samples in 60 min. This is a promising alternative method for the routinely diagnosis of Pneumocystis pneumonia.es
dc.formatapplication/pdfes
dc.format.extent14es
dc.language.isoenges
dc.publisherMDPIes
dc.relation.ispartofJournal of Fungi, 6 (4), 1-14.
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectNanoporous anodic aluminaes
dc.subjectPneumocystis jiroveciies
dc.subjectMolecular gateses
dc.subjectOligonucleotideses
dc.subjectBiosensores
dc.titleTriplex Hybridization-Based Nanosystem for the Rapid Screening of Pneumocystis Pneumonia in Clinical Sampleses
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Medicinaes
dc.identifier.doi10.3390/jof6040292es
dc.contributor.groupUniversidad de Sevilla. CTS-458: Investigación Clínica y Básica en Medicina Interna, Calidad y Continuidad Asistenciales
dc.journaltitleJournal of Fungies
dc.publication.volumen6es
dc.publication.issue4es
dc.publication.initialPage1es
dc.publication.endPage14es

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