Artículo
Bayesian Analysis of the Effects of Olive Oil-Derived Antioxidants on Cryopreserved Buck Sperm Parameters
Autor/es | Arando Arbulu, Ander
Navas González, Francisco Javier Bermúdez-Oria, Alejandra Delgado Bermejo, Juan Vicente Fernández Prior, África ![]() ![]() ![]() ![]() ![]() ![]() Pérez-Marín, Carlos Carmelo |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología |
Fecha de publicación | 2021 |
Fecha de depósito | 2023-11-29 |
Publicado en |
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Resumen | The present study evaluates the effect of olive oil-derived antioxidants, hydroxytyrosol
(HT) and 3,4-dihydroxyphenylglycol (DHPG), on cryopreserved caprine sperm using Bayesian in-
ference of ANOVA. For this proposal, ... The present study evaluates the effect of olive oil-derived antioxidants, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), on cryopreserved caprine sperm using Bayesian in- ference of ANOVA. For this proposal, sperm was collected, pooled and diluted in freezing media supplemented with different concentrations of HT, DHPG and the mixture (MIX) of both antioxidants. Sperm motility, viability, acrosome integrity, mitochondrial status, and lipid peroxidation (LPO) were assessed in fresh and frozen-thawed sperm samples. The results provided evidence that HT at low concentrations improves sperm motility and viability, and reduces the LPO. Contrastingly, DHPG and MIX exert a positive effect by reducing LPO values as concentration increases. Additionally, mi- tochondrial potential was reduced when samples were supplemented with HT at low concentrations and mixture of both antioxidants. Conclusively, the addition of olive oil-derived antioxidants (HT at 10 μg/mL and DHPG at 30 μg/mL) implements a protective effect in cryopreserved buck sperm. Bayesian analysis alternatives offer new possibilities to determine the repercussion of antioxidants on sperm, both quantitatively and qualitatively. |
Cita | Arando Arbulu, A., Navas González, .J., Bermúdez-Oria, A., Delgado Bermejo, J.V., Fernández Prior, Á. y Pérez-Marín, C.C. (2021). Bayesian Analysis of the Effects of Olive Oil-Derived Antioxidants on Cryopreserved Buck Sperm Parameters. Animals, 11 (7), 2032. https://doi.org/10.3390/ani11072032. |