Artículo
Inflammatory response in human lung cells stimulated with plasma from COPD patients
Autor/es | Arellano Orden, Elena
Calero Acuña, Carmen Sánchez López, Verónica Carrasco Hernández, Laura Márquez Martín, Eduardo Ortega Ruiz, Francisco Otero Candelera, Remedios Marín Hinojosa, Carmen López-Campos Bodineau, José Luis |
Departamento | Universidad de Sevilla. Departamento de Medicina |
Fecha de publicación | 2022 |
Fecha de depósito | 2023-11-20 |
Publicado en |
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Resumen | Background: Chronic obstructive pulmonary disease (COPD) is a condition resulting from a persistent inflammatory
state in the airways even after smoking cessation. Intriguingly, the reasons behind this persistence of the ... Background: Chronic obstructive pulmonary disease (COPD) is a condition resulting from a persistent inflammatory state in the airways even after smoking cessation. Intriguingly, the reasons behind this persistence of the inflammatory influx without smoking exposure have not been fully unraveled. We aimed to explore the hypothesis that systemic inflammation in COPD patients influences lung cell inflammatory response. Methods: We cultured human lung fibroblast and human airway epithelial cell lines with plasma from COPD patients (four emphysematous-COPD, four asthma-COPD overlap, four chronic bronchitis-COPD, and four bronchiectasis COPD), and four smokers or ex-smokers without COPD as controls. Non-stimulated cells were used as controls. We measured Interleukine-8 (IL-8), C-reactive protein (CRP) and matrix metalloproteinase-9 (MMP-9) in plasma and cul ture supernatants by ELISA. Results: Cells stimulated with plasma from COPD patients and non-COPD smoker subjects produced higher CRP, IL 8 and MMP-9 levels, an increase for COPD in CRP (p=0.029) in epithelial cells and IL-8 (p=0.039) in fibroblasts and decrease for MMP-9 (p=0.039) in fibroblasts, compared with non-stimulated cells. The response was higher in epithe lial cells for IL-8 (p=0.003) and in fibroblasts for MMP-9 (p=0.063). The plasma from chronic bronchitis and bronchiectasis phenotypes induced higher IL-8 in fibroblasts. Conclusions: Plasma from COPD patients increases the inflammatory response in lung epithelial cells and lung fibroblasts, with a different response depending on the cell type and clinical phenotype. |
Cita | Arellano Orden, E., Calero Acuña, C., Sánchez López, V., Carrasco Hernández, L., Márquez Martín, E., Ortega Ruiz, F.,...,López-Campos Bodineau, J.L. (2022). Inflammatory response in human lung cells stimulated with plasma from COPD patients. Multidisciplinary Respiratory Medicine, 17, 817. https://doi.org/10.4081/mrm.2022.817. |
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