dc.creator | Ortiz de la Rosa, José Manuel | es |
dc.creator | Rodríguez-Villodres, Ángel | es |
dc.creator | Gimeno Gascón, María Adelina | es |
dc.creator | Martín-Gutiérrez, Guillermo | es |
dc.creator | Cisneros, José Miguel | es |
dc.creator | Lepe Jiménez, José Antonio | es |
dc.date.accessioned | 2023-09-19T13:46:35Z | |
dc.date.available | 2023-09-19T13:46:35Z | |
dc.date.issued | 2023-02-14 | |
dc.identifier.citation | Ortiz de la Rosa, J.M., Rodríguez-Villodres, Á., Gimeno Gascón, M.A., Martín-Gutiérrez, G., Cisneros, J.M. y Lepe Jiménez, J.A. (2023). Rapid Detection of Piperacillin-Tazobactam Resistance in Klebsiella pneumoniae and Escherichia coli. MICROBIOLOGY SPECTRUM, 11 (2), e0436622. https://doi.org/10.1128/spectrum.04366-22. | |
dc.identifier.issn | 2165-0497 | es |
dc.identifier.uri | https://hdl.handle.net/11441/149017 | |
dc.description.abstract | Rapid determination of susceptibility to piperacillin-tazobactam (TZP) is very
important since the development of antibiotic resistance and inadequate treatment could
increase the risk of clinical failure in infected patients, especially if such resistance is
unknown to the clinician. Therefore, based on color change from orange to yellow of
phenol red due to glucose metabolism (bacterial growth) in the presence of an adequate
concentration of TZP (10 mg/L piperacillin and 5 mg/L tazobactam), the RapidTZP test
has been developed to detect TZP resistance in Escherichia coli and Klebsiella pneumoniae
isolates in a maximum of 3 h. A total of 140 isolates, 43 of E. coli and 97 of K. pneumoniae, were used to evaluate the performance of the test, 60 being resistant to TZP. The
sensitivity and specificity of the test were 98.24% and 100%, respectively. Additionally,
the RapidTZP test was validated by a pellet obtained directly from blood culture bottles.
A total of 37 positive blood cultures for E. coli and 43 for K. pneumoniae were used for
validation, 8 of them resistant to TZP. The sensitivity and specificity shown in the evaluation were 100% for both parameters. This new test is easy, fast, and accurate, providing
results in 3 h. | es |
dc.format | application/pdf | es |
dc.format.extent | 6 p. | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | MICROBIOLOGY SPECTRUM, 11 (2), e0436622. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Piperacillin-tazobactam | es |
dc.subject | Klebsiella pneumoniae | es |
dc.subject | Escherichia coli | es |
dc.subject | RapidTZP test | es |
dc.subject | Empirical therapy | es |
dc.title | Rapid Detection of Piperacillin-Tazobactam Resistance in Klebsiella pneumoniae and Escherichia coli | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.projectID | CD21/00098 | es |
dc.relation.projectID | JR20/00023 | es |
dc.relation.publisherversion | https://journals.asm.org/doi/10.1128/spectrum.04366-22 | es |
dc.identifier.doi | 10.1128/spectrum.04366-22 | es |
dc.journaltitle | MICROBIOLOGY SPECTRUM | es |
dc.publication.volumen | 11 | es |
dc.publication.issue | 2 | es |
dc.publication.initialPage | e0436622 | es |
dc.contributor.funder | Instituto de Salud Carlos III | es |
dc.contributor.funder | Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Ciencia, Innovación y Universidades | es |
dc.contributor.funder | JR19/00039 | es |