dc.creator | García-Domínguez, Daniel J. | es |
dc.creator | Hontecillas-Prieto, Lourdes | es |
dc.creator | Andrés León, Eduardo | es |
dc.creator | Sánchez-Molina, Sara | es |
dc.creator | Rodríguez-Núñez, Pablo | es |
dc.creator | Morón, Francisco J. | es |
dc.creator | Hajji, Nabil | es |
dc.creator | Mackintosh, Carlos | es |
dc.creator | Álava Casado, Enrique de | es |
dc.date.accessioned | 2023-06-27T09:54:13Z | |
dc.date.available | 2023-06-27T09:54:13Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | García-Domínguez, D.J., Hontecillas-Prieto, L., Andrés León, E., Sánchez-Molina, S., Rodríguez-Núñez, P., Morón, F.J.,...,Álava Casado, E.d. (2020). An inducible ectopic expression system of EWSR1-FLI1 as a tool for understanding Ewing sarcoma oncogénesis. PLoS ONE, 15 (6), e0234243. https://doi.org/10.1371/journal.pone.0234243. | |
dc.identifier.issn | 1932-6203 | es |
dc.identifier.uri | https://hdl.handle.net/11441/147502 | |
dc.description.abstract | The presence of the chimeric EWSR1-FLI1 oncoprotein is the main and initiating event defining Ewing sarcoma (ES). The dysregulation of epigenomic and proteomic homeostasis induced by the oncoprotein contributes to a wide variety of events involved in oncogenesis and tumor progression. Attempts at studying the effects of EWSR1-FLI1 in non-tumor cells to understand the mechanisms underlying sarcomagenesis have been unsuccessful to date, as ectopic expression of EWSR1-FLI1 blocks cell cycle progression and induces apoptosis in the tested cell lines. Therefore, it is essential to find a permissive cell type for EWSR1-FLI1 expression that allows its endogenous molecular functions to be studied. Here we have demonstrated that HeLa cell lines are permissive to EWSR1-FLI1 ectopic expression, and that our model substantially recapitulates the endogenous activity of the EWSR1-FLI1 fusion protein. This model could contribute to better understanding ES sarcomagenesis by helping to understand the molecular mechanisms induced by the EWSR1-FLI1 oncoprotein. | es |
dc.format | application/pdf | es |
dc.format.extent | 14 pág. | es |
dc.language.iso | eng | es |
dc.publisher | Public Library of Science | es |
dc.relation.ispartof | PLoS ONE, 15 (6), e0234243. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Growth | es |
dc.subject | Gene | es |
dc.subject | Initiation | es |
dc.subject | EWS-FLI1 | es |
dc.title | An inducible ectopic expression system of EWSR1-FLI1 as a tool for understanding Ewing sarcoma oncogénesis | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Citología e Histología Normal y Patológica | es |
dc.relation.projectID | CB16/12/00361 | es |
dc.relation.projectID | PI-0197-2016; PI-0013-2018 | es |
dc.relation.projectID | ID278742 EUROSARC | es |
dc.relation.projectID | PI11700464; RD06/0020/0059 | es |
dc.relation.publisherversion | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0234243 | es |
dc.identifier.doi | 10.1371/journal.pone.0234243 | es |
dc.journaltitle | PLoS ONE | es |
dc.publication.volumen | 15 | es |
dc.publication.issue | 6 | es |
dc.publication.initialPage | e0234243 | es |
dc.contributor.funder | Asociacion Espanola Contra el Cancer (AECC) | es |
dc.contributor.funder | CIBERONC | es |
dc.contributor.funder | Consejeria de Salud, Junta de Andalucia | es |
dc.contributor.funder | European Commission (FP7HEALTH-2011-two-stage) | es |
dc.contributor.funder | Ministry of Economy and Competitiveness of Spain-FEDER (CIBERONC) | es |