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dc.creatorGarcía-Domínguez, Daniel J.es
dc.creatorHontecillas-Prieto, Lourdeses
dc.creatorAndrés León, Eduardoes
dc.creatorSánchez-Molina, Saraes
dc.creatorRodríguez-Núñez, Pabloes
dc.creatorMorón, Francisco J.es
dc.creatorHajji, Nabiles
dc.creatorMackintosh, Carloses
dc.creatorÁlava Casado, Enrique dees
dc.date.accessioned2023-06-27T09:54:13Z
dc.date.available2023-06-27T09:54:13Z
dc.date.issued2020
dc.identifier.citationGarcía-Domínguez, D.J., Hontecillas-Prieto, L., Andrés León, E., Sánchez-Molina, S., Rodríguez-Núñez, P., Morón, F.J.,...,Álava Casado, E.d. (2020). An inducible ectopic expression system of EWSR1-FLI1 as a tool for understanding Ewing sarcoma oncogénesis. PLoS ONE, 15 (6), e0234243. https://doi.org/10.1371/journal.pone.0234243.
dc.identifier.issn1932-6203es
dc.identifier.urihttps://hdl.handle.net/11441/147502
dc.description.abstractThe presence of the chimeric EWSR1-FLI1 oncoprotein is the main and initiating event defining Ewing sarcoma (ES). The dysregulation of epigenomic and proteomic homeostasis induced by the oncoprotein contributes to a wide variety of events involved in oncogenesis and tumor progression. Attempts at studying the effects of EWSR1-FLI1 in non-tumor cells to understand the mechanisms underlying sarcomagenesis have been unsuccessful to date, as ectopic expression of EWSR1-FLI1 blocks cell cycle progression and induces apoptosis in the tested cell lines. Therefore, it is essential to find a permissive cell type for EWSR1-FLI1 expression that allows its endogenous molecular functions to be studied. Here we have demonstrated that HeLa cell lines are permissive to EWSR1-FLI1 ectopic expression, and that our model substantially recapitulates the endogenous activity of the EWSR1-FLI1 fusion protein. This model could contribute to better understanding ES sarcomagenesis by helping to understand the molecular mechanisms induced by the EWSR1-FLI1 oncoprotein.es
dc.formatapplication/pdfes
dc.format.extent14 pág.es
dc.language.isoenges
dc.publisherPublic Library of Sciencees
dc.relation.ispartofPLoS ONE, 15 (6), e0234243.
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectGrowthes
dc.subjectGenees
dc.subjectInitiationes
dc.subjectEWS-FLI1es
dc.titleAn inducible ectopic expression system of EWSR1-FLI1 as a tool for understanding Ewing sarcoma oncogénesises
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunologíaes
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Citología e Histología Normal y Patológicaes
dc.relation.projectIDCB16/12/00361es
dc.relation.projectIDPI-0197-2016; PI-0013-2018es
dc.relation.projectIDID278742 EUROSARCes
dc.relation.projectIDPI11700464; RD06/0020/0059es
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0234243es
dc.identifier.doi10.1371/journal.pone.0234243es
dc.journaltitlePLoS ONEes
dc.publication.volumen15es
dc.publication.issue6es
dc.publication.initialPagee0234243es
dc.contributor.funderAsociacion Espanola Contra el Cancer (AECC)es
dc.contributor.funderCIBERONCes
dc.contributor.funderConsejeria de Salud, Junta de Andaluciaes
dc.contributor.funderEuropean Commission (FP7HEALTH-2011-two-stage)es
dc.contributor.funderMinistry of Economy and Competitiveness of Spain-FEDER (CIBERONC)es

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