dc.creator | Velasco-Ortega, Eugenio | es |
dc.creator | Fos-Parra, Isabel | es |
dc.creator | Cabanillas Balsera, Daniel | es |
dc.creator | Gil, Javier | es |
dc.creator | Ortiz García, Iván | es |
dc.creator | Giner García, Mercedes | es |
dc.creator | Bocio-Núñez, Jesús | es |
dc.creator | Montoya García, María José | es |
dc.creator | Jiménez Guerra, Álvaro | es |
dc.date.accessioned | 2023-06-23T13:01:35Z | |
dc.date.available | 2023-06-23T13:01:35Z | |
dc.date.issued | 2023-02-09 | |
dc.identifier.citation | Velasco-Ortega, E., Fos-Parra, I., Cabanillas Balsera, D., Gil, J., Ortiz García, I., Giner García, M.,...,Jiménez Guerra, Á. (2023). Osteoblastic cell behavior and gene expression related to bone metabolism on different titanium surfaces. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (4), 3523. https://doi.org/10.3390/ijms24043523. | |
dc.identifier.issn | 1422-0067 | es |
dc.identifier.uri | https://hdl.handle.net/11441/147446 | |
dc.description.abstract | The surface topography of titanium dental implants has a great influence on osseointegration. In this work, we try to determine the osteoblastic behavior and gene expression of cells with
different titanium surfaces and relate them to the physicochemical properties of the surface. For this
purpose, we have used commercial titanium discs of grade 3: as-received corresponds to machined
titanium without any surface treatment (MA), chemically acid etched (AE), treated via sand blasting
with Al2O3 particles (SB) and a sand-blasting treatment with acid etching (SB+AE). The surfaces have
been observed using scanning electron microscopy (SEM) and the roughness, wettability and surface
energy with dispersive and polar components have been characterized. Osteoblastic cultures were
performed with SaOS-2 osteoblastic cells determining cell viability as well as alkaline phosphatase
levels for 3 and 21 days, and osteoblastic gene expression was determined. The roughness values of
the MA discs was 0.02 µm, which increases to 0.3 µm with acid attack and becomes the maximum for
the sand-blasted samples, reaching values of 1.2 µm for SB and SB+AE. The hydrophilic behavior of
the MA and AE samples with contact angles of 63◦ and 65◦
is superior to that of the rougher samples,
being 75◦
for SB and 82◦
for SB+AE. In all cases, they show good hydrophilicity. GB and GB+AE
surfaces present a higher polar component in the surface energy values, 11.96 and 13.18 mJ/m2
,
respectively, than AE and MA, 6.64 and 9.79 mJ/m2
, respectively. The osteoblastic cell viability values
at three days do not show statistically significant differences between the four surfaces. However, the
viability of the SB and SB+AE surfaces at 21 days is much higher than that of the AE and MA samples.
From the alkaline phosphatase studies, higher values were observed for those treated with sand blasting with and without acid etching compared to the other two surfaces, indicating a greater activity in
osteoblastic differentiation. In all cases except in the Osterix (Ostx) —osteoblast-specific transcription
factor—a decrease in gene expression is observed in relation to the MA samples (control). The most
important increase was observed for the SB+AE condition. A decrease in the gene expression of
Osteoprotegerine (OPG), Runt-related transcription factor 2 (Runx2), Receptor Activator of NF-κB
Ligand (RANKL) and Alkaline Phosphatase (Alp) genes was observed in the AE surface. | es |
dc.format | application/pdf | es |
dc.format.extent | 14 p. | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24 (4), 3523. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Titanium | es |
dc.subject | Surfaces | es |
dc.subject | Osteoblasts | es |
dc.subject | Gene expression | es |
dc.subject | Roughness and wettability | es |
dc.subject | Cell viability | es |
dc.title | Osteoblastic cell behavior and gene expression related to bone metabolism on different titanium surfaces | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Estomatología | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Citología e Histología Normal y Patológica | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.relation.projectID | RTI2018-098075-B-C21 | es |
dc.relation.projectID | RTI2018-098075-B-C22 | es |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/24/4/3523 | es |
dc.identifier.doi | 10.3390/ijms24043523 | es |
dc.journaltitle | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | es |
dc.publication.volumen | 24 | es |
dc.publication.issue | 4 | es |
dc.publication.initialPage | 3523 | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación. España | es |