Artículo
Salmonella Type III Secretion Effector SrfJ: A Glucosylceramidase Affecting the Lipidome and the Transcriptome of Mammalian Host Cells
Autor/es | Aguilera Herce, Julia
Panadero Medianero, Concepción Sánchez Romero, María Antonia Balbontín Soria, Roberto Bernal Bayard, Joaquín Ramos Morales, Francisco |
Departamento | Universidad de Sevilla. Departamento de Genética Universidad de Sevilla. Departamento de Microbiología y Parasitología |
Fecha de publicación | 2023 |
Fecha de depósito | 2023-05-10 |
Publicado en |
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Premios | Premio Mensual Publicación Científica Destacada de la US. Facultad de Biología |
Resumen | Type III secretion systems are found in many Gram-negative pathogens and symbionts of animals and plants. Salmonella enterica has two type III secretion systems associated with virulence, one involved in the invasion of ... Type III secretion systems are found in many Gram-negative pathogens and symbionts of animals and plants. Salmonella enterica has two type III secretion systems associated with virulence, one involved in the invasion of host cells and another involved in maintaining an appropriate intracellular niche. SrfJ is an effector of the second type III secretion system. In this study, we explored the biochemical function of SrfJ and the consequences for mammalian host cells of the expression of this S. enterica effector. Our experiments suggest that SrfJ is a glucosylceramidase that alters the lipidome and the transcriptome of host cells, both when expressed alone in epithelial cells and when translocated into macrophages in the context of Salmonella infection. We were able to identify seventeen lipids with higher levels and six lipids with lower levels in the presence of SrfJ. Analysis of the forty-five genes, the expression of which is significantly altered by SrfJ with a fold-change threshold of two, suggests that this effector may be involved in protecting Salmonella from host immune defenses. |
Agencias financiadoras | European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) Generalitat de Catalunya |
Identificador del proyecto | IU16-015805 |
Cita | Aguilera Herce, J., Panadero Medianero, C., Sánchez Romero, M.A., Balbontín Soria, R., Bernal Bayard, J. y Ramos Morales, F. (2023). Salmonella Type III Secretion Effector SrfJ: A Glucosylceramidase Affecting the Lipidome and the Transcriptome of Mammalian Host Cells. International Journal of Molecular Sciences, 24 (9), 8403. https://doi.org/10.3390/ijms24098403. |
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ijms-24-08403-v2.pdf | 3.941Mb | [PDF] | Ver/ | |