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dc.creatorVila Domínguez, Andreaes
dc.creatorCarretero Ledesma, Martaes
dc.creatorInfante Domínguez, Carmenes
dc.creatorCisneros, José Migueles
dc.creatorLepe Jiménez, José Antonioes
dc.creatorSmani, Youneses
dc.date.accessioned2023-05-09T12:41:29Z
dc.date.available2023-05-09T12:41:29Z
dc.date.issued2023-01-12
dc.identifier.citationVila Domínguez, A., Carretero Ledesma, M., Infante Domínguez, C., Cisneros, J.M., Lepe Jiménez, J.A. y Smani, Y. (2023). In vitro and in vivo virulence study of Listeria monocytogenes isolated from the Andalusian outbreak in 2019. TROPICAL MEDICINE AND INFECTIOUS DISEASE, 8 (1), 52. https://doi.org/10.3390/tropicalmed8010058.
dc.identifier.issn2414-6366es
dc.identifier.urihttps://hdl.handle.net/11441/145718
dc.description.abstractIn 2019, the biggest listeriosis outbreak by Listeria monocytogenes (Lm) in the South of Spain was reported, resulting in the death of three patients from 207 confirmed cases. One strain, belonging to clonal complex 388 (Lm CC388), has been isolated. We aimed to determine the Lm CC388 virulence in comparison with other highly virulent clones such as Lm CC1 and Lm CC4, in vitro and in vivo. Four L. monocytogenes strains (Lm CC388, Lm CC1, Lm CC4 and ATCC 19115) were used. Attachment to human lung epithelial cells (A549 cells) by these strains was characterized by adherence and invasion assays. Their cytotoxicities to A549 cells were evaluated by determining the cells viability. Their hemolysis activity was determined also. A murine intravenous infection model using these was performed to determine the concentration of bacteria in tissues and blood. Lm CC388 interaction with A549 cells is non-significantly higher than that of ATCC 19115 and Lm CC1, and lower than that of Lm CC4. Lm CC388 cytotoxicity is higher than that of ATCC 19115 and Lm CC1, and lower than that of Lm CC4. Moreover, Lm CC388 hemolysis activity is lower than that of the Lm CC4 strain, and higher than that of Lm CC1. Finally, in the murine intravenous infection model by Lm CC388, higher bacterial loads in tissues and at similar levels of Lm CC4 were observed. Although a lower rate of mortality of patients during the listeriosis outbreak in Spain in 2019 has been reported, the Lm CC388 strain has shown a greater or similar pathogenicity level in vitro and in an animal model, like Lm CC1 and Lm CC4.es
dc.formatapplication/pdfes
dc.format.extent9 p.es
dc.language.isoenges
dc.publisherMDPIes
dc.relation.ispartofTROPICAL MEDICINE AND INFECTIOUS DISEASE, 8 (1), 52.
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectListeriaes
dc.subjectOutbreakes
dc.subjectAnimal modeles
dc.subjectVirulencees
dc.subjectPathogenicityes
dc.titleIn vitro and in vivo virulence study of Listeria monocytogenes isolated from the Andalusian outbreak in 2019es
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Medicinaes
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiologíaes
dc.relation.projectIDCB21/13/00006es
dc.relation.publisherversionhttps://www.mdpi.com/2414-6366/8/1/58es
dc.identifier.doi10.3390/tropicalmed8010058es
dc.journaltitleTROPICAL MEDICINE AND INFECTIOUS DISEASEes
dc.publication.volumen8es
dc.publication.issue1es
dc.publication.initialPage52es
dc.contributor.funderConsejería de Salud. Junta de Andalucíaes

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