dc.creator | Vila Domínguez, Andrea | es |
dc.creator | Carretero Ledesma, Marta | es |
dc.creator | Infante Domínguez, Carmen | es |
dc.creator | Cisneros, José Miguel | es |
dc.creator | Lepe Jiménez, José Antonio | es |
dc.creator | Smani, Younes | es |
dc.date.accessioned | 2023-05-09T12:41:29Z | |
dc.date.available | 2023-05-09T12:41:29Z | |
dc.date.issued | 2023-01-12 | |
dc.identifier.citation | Vila Domínguez, A., Carretero Ledesma, M., Infante Domínguez, C., Cisneros, J.M., Lepe Jiménez, J.A. y Smani, Y. (2023). In vitro and in vivo virulence study of Listeria monocytogenes isolated from the Andalusian outbreak in 2019. TROPICAL MEDICINE AND INFECTIOUS DISEASE, 8 (1), 52. https://doi.org/10.3390/tropicalmed8010058. | |
dc.identifier.issn | 2414-6366 | es |
dc.identifier.uri | https://hdl.handle.net/11441/145718 | |
dc.description.abstract | In 2019, the biggest listeriosis outbreak by Listeria monocytogenes (Lm) in the South of Spain
was reported, resulting in the death of three patients from 207 confirmed cases. One strain, belonging
to clonal complex 388 (Lm CC388), has been isolated. We aimed to determine the Lm CC388 virulence
in comparison with other highly virulent clones such as Lm CC1 and Lm CC4, in vitro and in vivo.
Four L. monocytogenes strains (Lm CC388, Lm CC1, Lm CC4 and ATCC 19115) were used. Attachment
to human lung epithelial cells (A549 cells) by these strains was characterized by adherence and
invasion assays. Their cytotoxicities to A549 cells were evaluated by determining the cells viability.
Their hemolysis activity was determined also. A murine intravenous infection model using these
was performed to determine the concentration of bacteria in tissues and blood. Lm CC388 interaction
with A549 cells is non-significantly higher than that of ATCC 19115 and Lm CC1, and lower than that
of Lm CC4. Lm CC388 cytotoxicity is higher than that of ATCC 19115 and Lm CC1, and lower than
that of Lm CC4. Moreover, Lm CC388 hemolysis activity is lower than that of the Lm CC4 strain, and
higher than that of Lm CC1. Finally, in the murine intravenous infection model by Lm CC388, higher
bacterial loads in tissues and at similar levels of Lm CC4 were observed. Although a lower rate of
mortality of patients during the listeriosis outbreak in Spain in 2019 has been reported, the Lm CC388
strain has shown a greater or similar pathogenicity level in vitro and in an animal model, like Lm
CC1 and Lm CC4. | es |
dc.format | application/pdf | es |
dc.format.extent | 9 p. | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | TROPICAL MEDICINE AND INFECTIOUS DISEASE, 8 (1), 52. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Listeria | es |
dc.subject | Outbreak | es |
dc.subject | Animal model | es |
dc.subject | Virulence | es |
dc.subject | Pathogenicity | es |
dc.title | In vitro and in vivo virulence study of Listeria monocytogenes isolated from the Andalusian outbreak in 2019 | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.projectID | CB21/13/00006 | es |
dc.relation.publisherversion | https://www.mdpi.com/2414-6366/8/1/58 | es |
dc.identifier.doi | 10.3390/tropicalmed8010058 | es |
dc.journaltitle | TROPICAL MEDICINE AND INFECTIOUS DISEASE | es |
dc.publication.volumen | 8 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | 52 | es |
dc.contributor.funder | Consejería de Salud. Junta de Andalucía | es |