Article
Impact of “chemical cocktails” exposure in shaping mice gut microbiota and the role of selenium supplementation combining metallomics, metabolomics, and metataxonomics
Author/s | Arias Borrego, Ana
Selma Royo, Marta Collado, María Carmen Abril, Nieves García Barrera, Tamara |
Department | Universidad de Sevilla. Departamento de Química Analítica |
Publication Date | 2022 |
Deposit Date | 2023-05-04 |
Published in |
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Abstract | Biological systems are exposed to a complex environment in which pollutants can interact through synergistic or antagonistic mechanisms, but limited information is available on the combined effects. To this end, conventional ... Biological systems are exposed to a complex environment in which pollutants can interact through synergistic or antagonistic mechanisms, but limited information is available on the combined effects. To this end, conventional and antibiotic-treated (Abx) mice models were fed regular rodent or selenium (Se) supplemented diets and exposed to a “chemical cocktail” (CC) including metals and pharmaceuticals. Metallomics, metabolomics, and metataxomics were combined to delve into the impact on gut microbiota, plasma selenoproteome, metabolome, and arsenic metabolization. At the molecular level, Se decreased the concentration of the antioxidant glutathione peroxidase in plasma and increased the arsenic methylation rate, possibly favoring its excretion, but not in the Abx and also plasma metabolomes of Abx, and Abx-Se were not differentiated. Moreover, numerous associations were obtained between plasma selenoproteins and gut microbes. Se-supplementation partially antagonizes the gut microbiota alteration caused by Abx, and slightly by CC, but strongly altered profiles were observed in CC-Abx-Se, suggesting synergistic deleterious effects between pollutants, Abx and Se. Moreover, although CC and Abx changed gut microbiota, several common taxa were enriched in CC-Abx and control mice, indicating possible synergistic effects. Our results suggest a potential beneficial impact of supplementation, but mediated by gut microbes being reversed in their absence. |
Funding agencies | Ministerio de Ciencia e Innovación (MICIN). España Junta de Andalucía European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) Universidad de Huelva Fundación Ramón Areces |
Project ID. | PG2018–096608-B-C21
UHU-1256905 UHU- 202009 UNHU13–1E-1611 CIVP19A5918 |
Citation | Arias Borrego, A., Selma Royo, M., Collado, M.C., Abril, N. y García Barrera, T. (2022). Impact of “chemical cocktails” exposure in shaping mice gut microbiota and the role of selenium supplementation combining metallomics, metabolomics, and metataxonomics. Journal of Hazardous Materials, 438. https://doi.org/10.1016/j.jhazmat.2022.129444. |
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