dc.creator | Lewinska, Monika | es |
dc.creator | Kårhus, Martin Lund | es |
dc.creator | Ellegaard, Anne-Marie Gade | es |
dc.creator | Romero Gómez, Manuel | es |
dc.creator | Macias, Rocio I R | es |
dc.creator | Andersen, Jesper B. | es |
dc.creator | Knop, Filip Krag | es |
dc.date.accessioned | 2023-04-24T08:09:24Z | |
dc.date.available | 2023-04-24T08:09:24Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Lewinska, M., Kårhus, M.L., Ellegaard, A.G., Romero Gómez, M., Macias, R.I.R., Andersen, J.B. y Knop, F.K. (2023). Serum lipidome unravels a diagnostic potential in bile acid diarrhoea. Gut, 1-11. https://doi.org/10.1136/gutjnl-2022-329213. | |
dc.identifier.issn | 0017-5749 | es |
dc.identifier.issn | 1468-3288 online | es |
dc.identifier.uri | https://hdl.handle.net/11441/144781 | |
dc.description.abstract | Objective: Bile acid diarrhoea (BAD) is debilitating yet treatable, but it remains underdiagnosed due to challenging diagnostics. We developed a blood test-based method to guide BAD diagnosis.
Design: We included serum from 50 treatment-naive patients with BAD diagnosed by gold standard 75selenium homotaurocholic acid test, 56 feature-matched controls and 37 patients with non-alcoholic fatty liver disease (NAFLD). Metabolomes were generated using mass spectrometry covering 1295 metabolites and compared between groups. Machine learning was used to develop a BAD Diagnostic Score (BDS).
Results: Metabolomes of patients with BAD significantly differed from controls and NAFLD. We detected 70 metabolites with a discriminatory performance in the discovery set with an area under receiver-operating curve metric above 0.80. Logistic regression modelling using concentrations of decanoylcarnitine, cholesterol ester (22:5), eicosatrienoic acid, L-alpha-lysophosphatidylinositol (18:0) and phosphatidylethanolamine (O-16:0/18:1) distinguished BAD from controls with a sensitivity of 0.78 (95% CI 0.64 to 0.89) and a specificity of 0.93 (95% CI 0.83 to 0.98). The model was independent of covariates (age, sex, body mass index) and distinguished BAD from NAFLD irrespective of fibrosis stage. BDS outperformed other blood test-based tests (7-alpha-hydroxy-4-cholesten-3-one and fibroblast growth factor 19) currently under development.
Conclusions: BDS derived from serum metabolites in a single-blood sample showed robust identification of patients with BAD with superior specificity and sensitivity compared with current blood test-based diagnostics. | es |
dc.format | application/pdf | es |
dc.format.extent | 11 p. | es |
dc.language.iso | eng | es |
dc.relation.ispartof | Gut, 1-11. | |
dc.rights | Atribución-NoComercial 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | * |
dc.subject | Bile acid | es |
dc.subject | Diarrhoea | es |
dc.title | Serum lipidome unravels a diagnostic potential in bile acid diarrhoea | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.relation.publisherversion | https://gut.bmj.com/content/early/2023/04/18/gutjnl-2022-329213 | es |
dc.identifier.doi | 10.1136/gutjnl-2022-329213 | es |
dc.contributor.group | Universidad de Sevilla. CTS 532: Unidad de Hepatología | es |
dc.journaltitle | Gut | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 11 | es |