Article
Role of a cryptic tRNA gene operon in survival under translational stress
Author/s | Santamaría Gómez, Javier
Rubio, Miguel Ángel López Igual, Rocío Romero Losada, Ana Belén Delgado Chaves, Fernando M. Bru Martínez, Roque Romero Campero, Francisco José Herrero Moreno, Antonia Ibba, Michael Ochoa de Alda, Jesús A. G. Luque Romero, Ignacio |
Department | Universidad de Sevilla. Departamento de Ciencia de la Computación e Inteligencia Artificial |
Publication Date | 2021-08-11 |
Deposit Date | 2023-04-13 |
Published in |
|
Abstract | As compared to eukaryotes, bacteria have a reduced tRNA gene set encoding between 30 and 220 tR-NAs. Although in most bacterial phyla tRNA genes
are dispersed in the genome, many species from distinct phyla also show genes ... As compared to eukaryotes, bacteria have a reduced tRNA gene set encoding between 30 and 220 tR-NAs. Although in most bacterial phyla tRNA genes are dispersed in the genome, many species from distinct phyla also show genes forming arrays. Here, we show that two types of arrays with distinct evolutionary origins exist. This work focuses on long tRNA gene arrays (L-arrays) that encompass up to 43 genes, which disseminate by horizontal gene transfer and contribute supernumerary tRNA genes to the host. Although in the few cases previously studied these arrays were reported to be poorly transcribed, here we show that the L-array of the model cyanobacterium Anabaena sp. PCC 7120, encoding 23 functional tRNAs, is largely induced upon impairment of the translation machinery. The cellular response to this challenge involves a global reprogramming of the transcriptome in two phases. tRNAs encoded in the array are induced in the second phase of the response, directly contributing to cell survival. Results presented here show that in some bacteria the tRNA gene set may be partitioned between a housekeeping subset, which constantly sustains translation, and an inducible subset that is generally silent but can provide functionality under particular conditions. |
Funding agencies | Ministerio de Ciencia, Innovación y Universidades (MICINN). España Agencia Estatal de Investigación. España |
Project ID. | BFU2016-77097-P
BIO2017-84066-R PID2019-104784RJ-100/AEI/10.13039/501100011033 |
Citation | Santamaría Gómez, J., Rubio, M.Á., López Igual, R., Romero Losada, A.B., Delgado Chaves, F.M., Bru Martínez, R.,...,Luque Romero, I. (2021). Role of a cryptic tRNA gene operon in survival under translational stress. Nucleic Acids Research, 49 (15), 8757-8776. https://doi.org/10.1093/nar/gkab661. |
Files | Size | Format | View | Description |
---|---|---|---|---|
gkab661.pdf | 10.11Mb | [PDF] | View/ | |