Artículo
Chromosomal Position of Ribosomal Protein Genes Affects Long-Term Evolution of Vibrio cholerae
Autor/es | Larotonda, Leticia
Mornico, Damien Khanna, Varun Bernal Bayard, Joaquín Soler Bistue, Alfonso |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2023 |
Fecha de depósito | 2023-04-10 |
Publicado en |
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Resumen | It is unclear how gene order within the chromosome influences genome evolution. Bacteria cluster transcription and translation genes close to the replication origin (oriC). In Vibrio cholerae, relocation of s10-spc-α locus ... It is unclear how gene order within the chromosome influences genome evolution. Bacteria cluster transcription and translation genes close to the replication origin (oriC). In Vibrio cholerae, relocation of s10-spc-α locus (S10), the major locus of ribosomal protein genes, to ectopic genomic positions shows that its relative distance to the oriC correlates to a reduction in growth rate, fitness, and infectivity. To test the long-term impact of this trait, we evolved 12 populations of V. cholerae strains bearing S10 at an oriC-proximal or an oriC-distal location for 1,000 generations. During the first 250 generations, positive selection was the main force driving mutation. After 1,000 generations, we observed more nonadaptative mutations and hypermutator genotypes. Populations fixed inactivating mutations at many genes linked to virulence: flagellum, chemotaxis, biofilm, and quorum sensing. Throughout the experiment, all populations increased their growth rates. However, those bearing S10 close to oriC remained the fittest, indicating that suppressor mutations cannot compensate for the genomic position of the main ribosomal protein locus. Selection and sequencing of the fastest-growing clones allowed us to characterize mutations inactivating, among other sites, flagellum master regulators. Reintroduction of these mutations into the wild-type context led to a ≈10% growth improvement. In conclusion, the genomic location of ribosomal protein genes conditions the evolutionary trajectory of V. cholerae. While genomic content is highly plastic in prokaryotes, gene order is an underestimated factor that conditions cellular physiology and evolution. A lack of suppression enables artificial gene relocation as a tool for genetic circuit reprogramming. |
Agencias financiadoras | Centre national de la recherche scientifique (CNRS). France French National Research Agency (ANR). France International Centre for Genetic Engineering and Biotechnology (ICGEB) ECOS-SUD-Cooperación científica Francia América Latina Gobierno de Francia Agencia Nacional de Promoción de la Investigación, Desarrollo Tecnológico y la Innovación. Argentina |
Identificador del proyecto | UMR3525
ANR-10-BLAN593 131301 ANR-14-CE10-0007 CRP/ARG18-06_EC ECOS-SUD 18ST06 ANR-10-LABX-62-IBEID PICT-2017-0424 PICT-2020-0521 PICT-2018-0476 |
Cita | Larotonda, L., Mornico, D., Khanna, V., Bernal Bayard, J. y Soler Bistue, A. (2023). Chromosomal Position of Ribosomal Protein Genes Affects Long-Term Evolution of Vibrio cholerae. mBio. https://doi.org/10.1128/mbio.03432-22. |
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