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Spb4p, an essential putative RNA helicase, is required for a late step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae
dc.creator | Cruz Díaz, Jesús de la | es |
dc.creator | Kressler, Dieter | es |
dc.creator | Rojo, Manuel | es |
dc.creator | Tollervey, David W. | es |
dc.creator | Linder, Patrick | es |
dc.date.accessioned | 2023-03-24T15:38:24Z | |
dc.date.available | 2023-03-24T15:38:24Z | |
dc.date.issued | 1998 | |
dc.identifier.citation | Cruz Díaz, J.d.l., Kressler, D., Rojo, M., Tollervey, D.W. y Linder, P. (1998). Spb4p, an essential putative RNA helicase, is required for a late step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae. RNA, 4 (10), 1268-1281. https://doi.org/10.1017/S1355838298981158. | |
dc.identifier.issn | 1355-8382 | es |
dc.identifier.issn | 1469-9001 | es |
dc.identifier.uri | https://hdl.handle.net/11441/143567 | |
dc.description.abstract | Spb4p is a putative ATP-dependent RNA helicase that is required for synthesis of 60S ribosomal subunits. Polysome analyses of strains genetically depleted of Spb4p or carrying the cold-sensitive spb4-1 mutation revealed an under-accumulation of 60S ribosomal subunits. Analysis of pre-rRNA processing by pulse-chase labeling, northern hybridization, and primer extension indicated that these strains exhibited a reduced synthesis of the 25S/5.8S rRNAs, due to inhibition of processing of the 27SB pre-rRNAs. At later times of depletion of Spb4p or following transfer of the spb4-1 strain to more restrictive temperatures, the early pre-rRNA processing steps at sites A0, A1, and A2 were also inhibited. Sucrose gradient fractionation showed that the accumulated 27SB pre-rRNAs are associated with a high-molecular-weight complex, most likely the 66S pre-ribosomal particle. An HA epitope-tagged Spb4p is localized to the nucleolus and the adjacent nucleoplasmic area. On sucrose gradients, HA-Spb4p was found almost exclusively in rapidly sedimenting complexes and showed a peak in the fractions containing the 66S pre-ribosomes. We propose that Spb4p is involved directly in a late and essential step during assembly of 60S ribosomal subunits, presumably by acting as an rRNA helicase. | es |
dc.description.sponsorship | Swiss National Science Foundation 31-43321.95 | es |
dc.format | application/pdf | es |
dc.format.extent | 15 p. | es |
dc.language.iso | eng | es |
dc.publisher | Cold Spring Harbor Laboratory Press | es |
dc.relation.ispartof | RNA, 4 (10), 1268-1281. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | DEAD-box proteins | es |
dc.subject | Pre-rRNA processing | es |
dc.subject | Ribosome biogenesis | es |
dc.subject | Yeast | es |
dc.title | Spb4p, an essential putative RNA helicase, is required for a late step in the assembly of 60S ribosomal subunits in Saccharomyces cerevisiae | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | 31-43321.95 | es |
dc.relation.publisherversion | https://doi.org/10.1017/S1355838298981158 | es |
dc.identifier.doi | 10.1017/S1355838298981158 | es |
dc.journaltitle | RNA | es |
dc.publication.volumen | 4 | es |
dc.publication.issue | 10 | es |
dc.publication.initialPage | 1268 | es |
dc.publication.endPage | 1281 | es |
dc.contributor.funder | Swiss National Science Foundation (SNFS) | es |