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dc.creatorPazos, Irenees
dc.creatorPuig Tinto, Martaes
dc.creatorBetancur, Lauraes
dc.creatorCordero, Jorgees
dc.creatorJiménez Menéndez, Nereidaes
dc.creatorAbella, Marces
dc.creatorHernandez, Altair C.es
dc.creatorSabido Bozo, Susanaes
dc.creatorMuñiz Guinea, Manueles
dc.creatorGallego, Orioles
dc.date.accessioned2023-03-22T15:27:48Z
dc.date.available2023-03-22T15:27:48Z
dc.date.issued2023
dc.identifier.citationPazos, I., Puig Tinto, M., Betancur, L., Cordero, J., Jiménez Menéndez, N., Abella, M.,...,Gallego, O. (2023). The P4-ATPase Drs2 Interacts with and Stabilizes the Multisubunit Tethering Complex TRAPPIII in Yeast. EMBO Reports, e56134. https://doi.org/10.15252/embr.20225613.
dc.identifier.issn1469-221Xes
dc.identifier.issn1469-3178es
dc.identifier.urihttps://hdl.handle.net/11441/143525
dc.description.abstractMultisubunit Tethering Complexes (MTCs) are a set of conserved protein complexes that tether vesicles at the acceptor membrane. Interactions with other components of the trafficking machinery regulate MTCs through mechanisms that are partially understood. Here, we systematically investigate the interactome that regulates MTCs. We report that P4-ATPases, a family of lipid flippases, interact with MTCs that participate in the anterograde and retrograde transport at the Golgi, such as TRAPPIII. We use the P4-ATPase Drs2 as a paradigm to investigate the mechanism and biological relevance of this interplay during transport of Atg9 vesicles. Binding of Trs85, the sole-specific subunit of TRAPPIII, to the N-terminal tail of Drs2 stabilizes TRAPPIII on membranes loaded with Atg9 and is required for Atg9 delivery during selective autophagy, a role that is independent of P4-ATPase canonical functions. This mechanism requires a conserved I(S/R)TTK motif that also mediates the interaction of the P4-ATPases Dnf1 and Dnf2 with MTCs, suggesting a broader role of P4-ATPases in MTC regulation.es
dc.description.sponsorshipMinisterio de Economía y Competitividad PID2021-127773NB-I00; PGC2018-095745-B-I00; EUR2019-103815; MDM-2014-0370; BES-2013-063945es
dc.description.sponsorshipHuman Frontier Science Program RGP0017/2020es
dc.formatapplication/pdfes
dc.format.extent18 p.es
dc.language.isoenges
dc.publisherJohn Wiley & Sonses
dc.relation.ispartofEMBO Reports, e56134.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleThe P4-ATPase Drs2 Interacts with and Stabilizes the Multisubunit Tethering Complex TRAPPIII in Yeastes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Biología Celulares
dc.relation.projectIDPID2021-127773NB-I00es
dc.relation.projectIDPGC2018-095745-B-I00es
dc.relation.projectIDEUR2019-103815es
dc.relation.projectIDMDM-2014-0370es
dc.relation.projectIDBES-2013-063945es
dc.relation.projectIDRGP0017/2020es
dc.relation.publisherversionhttps://doi.org/10.15252/embr.202256134es
dc.identifier.doi10.15252/embr.202256134es
dc.journaltitleEMBO Reportses
dc.publication.initialPagee56134es
dc.contributor.funderMinisterio de Economía y Competitividad (MINECO). Españaes
dc.contributor.funderHuman Frontier Science Program (HFSP)es

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