Artículo
The P4-ATPase Drs2 Interacts with and Stabilizes the Multisubunit Tethering Complex TRAPPIII in Yeast
Autor/es | Pazos, Irene
Puig Tinto, Marta Betancur, Laura Cordero, Jorge Jiménez Menéndez, Nereida Abella, Marc Hernandez, Altair C. Sabido Bozo, Susana Muñiz Guinea, Manuel Gallego, Oriol |
Departamento | Universidad de Sevilla. Departamento de Biología Celular |
Fecha de publicación | 2023 |
Fecha de depósito | 2023-03-22 |
Publicado en |
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Resumen | Multisubunit Tethering Complexes (MTCs) are a set of conserved protein complexes that tether vesicles at the acceptor membrane. Interactions with other components of the trafficking machinery regulate MTCs through mechanisms ... Multisubunit Tethering Complexes (MTCs) are a set of conserved protein complexes that tether vesicles at the acceptor membrane. Interactions with other components of the trafficking machinery regulate MTCs through mechanisms that are partially understood. Here, we systematically investigate the interactome that regulates MTCs. We report that P4-ATPases, a family of lipid flippases, interact with MTCs that participate in the anterograde and retrograde transport at the Golgi, such as TRAPPIII. We use the P4-ATPase Drs2 as a paradigm to investigate the mechanism and biological relevance of this interplay during transport of Atg9 vesicles. Binding of Trs85, the sole-specific subunit of TRAPPIII, to the N-terminal tail of Drs2 stabilizes TRAPPIII on membranes loaded with Atg9 and is required for Atg9 delivery during selective autophagy, a role that is independent of P4-ATPase canonical functions. This mechanism requires a conserved I(S/R)TTK motif that also mediates the interaction of the P4-ATPases Dnf1 and Dnf2 with MTCs, suggesting a broader role of P4-ATPases in MTC regulation. |
Agencias financiadoras | Ministerio de Economía y Competitividad (MINECO). España Human Frontier Science Program (HFSP) |
Identificador del proyecto | PID2021-127773NB-I00
PGC2018-095745-B-I00 EUR2019-103815 MDM-2014-0370 BES-2013-063945 RGP0017/2020 |
Cita | Pazos, I., Puig Tinto, M., Betancur, L., Cordero, J., Jiménez Menéndez, N., Abella, M.,...,Gallego, O. (2023). The P4-ATPase Drs2 Interacts with and Stabilizes the Multisubunit Tethering Complex TRAPPIII in Yeast. EMBO Reports, e56134. https://doi.org/10.15252/embr.20225613. |
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The P4-ATPase.pdf | 2.733Mb | [PDF] | Ver/ | |