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dc.creatorRamos Sáenz, Anaes
dc.creatorGonzález Álvarez, Danieles
dc.creatorRodríguez Galán, Olgaes
dc.creatorRodríguez Gil, Alfonsoes
dc.creatorGaspar, Sonia G.es
dc.creatorVillalobo Polo, Eduardoes
dc.creatorDosil, Mercedeses
dc.creatorCruz Díaz, Jesús de laes
dc.date.accessioned2023-03-20T13:32:22Z
dc.date.available2023-03-20T13:32:22Z
dc.date.issued2019
dc.identifier.citationRamos Sáenz, A., González Álvarez, D., Rodríguez Galán, O., Rodríguez Gil, A., Gaspar, S.G., Villalobo Polo, E.,...,Cruz Díaz, J.d.l. (2019). Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae. RNA, 25 (11), 1561-1575. https://doi.org/10.1261/rna.072116.119.
dc.identifier.issn1469-9001es
dc.identifier.urihttps://hdl.handle.net/11441/143475
dc.description.abstractIn Saccharomyces cerevisiae, more than 250 trans-acting factors are involved in the maturation of 40S and 60S ribosomal subunits. The expression of most of these factors is transcriptionally coregulated to ensure correct ribosome production under a wide variety of environmental and intracellular conditions. Here, we identified the essential nucleolar Pol5 protein as a novel trans-acting factor required for the synthesis of 60S ribosomal subunits. Pol5 weakly and/or transiently associates with early to medium pre-60S ribosomal particles. Depletion of and temperature-sensitive mutations in Pol5 result in a deficiency of 60S ribosomal subunits and accumulation of half-mer polysomes. Both processing of 27SB pre-rRNA to mature 25S rRNA and release of pre-60S ribosomal particles from the nucle(ol)us to the cytoplasm are impaired in the Pol5-depleted strain. Moreover, we identified the genes encoding ribosomal proteins uL23 and eL27A as multicopy suppressors of the slow growth of a temperature-sensitive pol5 mutant. These results suggest that Pol5 could function in ensuring the correct folding of 25S rRNA domain III; thus, favoring the correct assembly of these two ribosomal proteins at their respective binding sites into medium pre-60S ribosomal particles. Pol5 is homologous to the human tumor suppressor Myb-binding protein 1A (MYBBP1A). However, expression of MYBBP1A failed to complement the lethal phenotype of a pol5 null mutant strain though interfered with 60S ribosomal subunit biogenesis.es
dc.formatapplication/pdfes
dc.format.extent16 p.es
dc.language.isoenges
dc.publisherCold Spring Harbor Laboratory Presses
dc.relation.ispartofRNA, 25 (11), 1561-1575.
dc.rightsAtribución-NoComercial 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectribosome biogenesises
dc.subjectpre-rRNA processinges
dc.subjectMYBBP1Aes
dc.subjectribosomal proteins uL23 and eL27es
dc.subjectnucleoluses
dc.titlePol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiaees
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiologíaes
dc.relation.projectIDBFU2016-75352-Pes
dc.relation.projectIDBFU2014-52729-Pes
dc.relation.projectIDBFU2017-88192-Pes
dc.relation.publisherversionhttps://dx.doi.org/10.1261/rna.072116.119es
dc.identifier.doi10.1261/rna.072116.119es
dc.journaltitleRNAes
dc.publication.volumen25es
dc.publication.issue11es
dc.publication.initialPage1561es
dc.publication.endPage1575es
dc.contributor.funderMinisterio de Economía y Competitividad (MINECO). Españaes
dc.contributor.funderEuropean Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)es
dc.contributor.funderAgencia Estatal de Investigación. Españaes

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