dc.creator | López Igual, Rocío | es |
dc.creator | Bernal Bayard, Joaquín | es |
dc.creator | Rodríguez Patón, Alfonso | es |
dc.creator | Ghigo, Jean Marc | es |
dc.creator | Mazel, Didier | es |
dc.date.accessioned | 2023-02-13T15:40:00Z | |
dc.date.available | 2023-02-13T15:40:00Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | López Igual, R., Bernal Bayard, J., Rodríguez Patón, A., Ghigo, J.M. y Mazel, D. (2019). Engineered toxin–intein antimicrobials can selectively target and kill antibiotic-resistant bacteria in mixed populations. Nature Biotechnology, 37 (7), 755-760. https://doi.org/10.1038/s41587-019-0105-3. | |
dc.identifier.issn | 1087-0156 | es |
dc.identifier.issn | 1546-1696 | es |
dc.identifier.uri | https://hdl.handle.net/11441/142672 | |
dc.description.abstract | Targeted killing of pathogenic bacteria without harming beneficial members of host microbiota holds promise as a strategy to cure disease and limit both antimicrobial-related dysbiosis and development of antimicrobial resistance. We engineer toxins that are split by inteins and deliver them by conjugation into a mixed population of bacteria. Our toxin–intein antimicrobial is only activated in bacteria that harbor specific transcription factors. We apply our antimicrobial to specifically target and kill antibiotic-resistant Vibrio cholerae present in mixed populations. We find that 100% of antibiotic-resistant V. cholerae receiving the plasmid are killed. Escape mutants were extremely rare (10−6–10−8). We show that conjugation and specific killing of targeted bacteria occurs in the microbiota of zebrafish and crustacean larvae, which are natural hosts for Vibrio spp. Toxins split with inteins could form the basis of precision antimicrobials to target pathogens that are antibiotic resistant. | es |
dc.description.sponsorship | Centre National de la Recherche Scientifique CNRS-UMR 3525 | es |
dc.description.sponsorship | French Government Investissement d'Avenir ANR-10-LABX-62-IBEID | es |
dc.description.sponsorship | Agencia Estatal de Investigación TIN2016-81079-R | es |
dc.description.sponsorship | Comunidad de Madrid B2017/BMD-3691 | es |
dc.description.sponsorship | Fondation pour la Recherche Médicale DBF20160635736, DEQ20140329508 | es |
dc.format | application/pdf | es |
dc.format.extent | 36 p. | es |
dc.language.iso | eng | es |
dc.publisher | Springer Nature | es |
dc.relation.ispartof | Nature Biotechnology, 37 (7), 755-760. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Engineered toxin–intein antimicrobials can selectively target and kill antibiotic-resistant bacteria in mixed populations | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/submittedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | CNRS-UMR 3525 | es |
dc.relation.projectID | ANR-10-LABX-62-IBEID | es |
dc.relation.projectID | TIN2016-81079-R | es |
dc.relation.projectID | B2017/BMD-3691 | es |
dc.relation.projectID | DBF20160635736 | es |
dc.relation.projectID | DEQ20140329508 | es |
dc.relation.publisherversion | https://doi.org/10.1038/s41587-019-0105-3 | es |
dc.identifier.doi | 10.1038/s41587-019-0105-3 | es |
dc.journaltitle | Nature Biotechnology | es |
dc.publication.volumen | 37 | es |
dc.publication.issue | 7 | es |
dc.publication.initialPage | 755 | es |
dc.publication.endPage | 760 | es |
dc.contributor.funder | Centre national de la recherche scientifique (CNRS). France | es |
dc.contributor.funder | French Government Investissement d'Avenir. France | es |
dc.contributor.funder | Agencia Estatal de Investigación. España | es |
dc.contributor.funder | Comunidad Autónoma de Madrid | es |
dc.contributor.funder | Fondation pour la Recherche Médicale. France | es |