Artículo
Selective ribosome profiling reveals a role for SecB in the co-translational inner membrane protein biogenesis
Autor/es | Eismann, Lena
Fijalkowski, Igor Galmozzi, Carla V. Koubek, Jiří Tippmann, Frank Van Damme, Petra Kramer, Günter |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2022 |
Fecha de depósito | 2023-01-19 |
Publicado en |
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Resumen | The chaperone SecB has been implicated in de novo protein folding and translocation across the membrane, but it remains unclear which nascent polypeptides SecB binds, when during translation SecB acts, how SecB function ... The chaperone SecB has been implicated in de novo protein folding and translocation across the membrane, but it remains unclear which nascent polypeptides SecB binds, when during translation SecB acts, how SecB function is coordinated with other chaperones and targeting factors, and how polypeptide engagement contributes to protein biogenesis. Using selective ribosome profiling, we show that SecB binds many nascent cytoplasmic and translocated proteins generally late during translation and controlled by the chaperone trigger factor. Revealing an uncharted role in co-translational translocation, inner membrane proteins (IMPs) are the most prominent nascent SecB interactors. Unlike other substrates, IMPs are bound early during translation, following the membrane targeting by the signal recognition particle. SecB remains bound until translation is terminated, and contributes to membrane insertion. Our study establishes a role of SecB in the co-translational maturation of proteins from all cellular compartments and functionally implicates cytosolic chaperones in membrane protein biogenesis. |
Agencias financiadoras | Deutsche Forschungsgemeinschaft / German Research Foundation (DFG) European Union (UE) Research Foundation Flanders |
Identificador del proyecto | DFG KR 3593/2-1
803972 823839 G051120N |
Cita | Eismann, L., Fijalkowski, I., Galmozzi, C.V., Koubek, J., Tippmann, F., Van Damme, P. y Kramer, G. (2022). Selective ribosome profiling reveals a role for SecB in the co-translational inner membrane protein biogenesis. Cell Reports, 41 (10), 111776. https://doi.org/10.1016/j.celrep.2022.111776. |
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