dc.creator | Nabi, Dalileh | es |
dc.creator | Drechsler, Hauke | es |
dc.creator | Pschirer, Johannes | es |
dc.creator | Korn, Franz | es |
dc.creator | Schuler, Nadine | es |
dc.creator | Diez, Stefan | es |
dc.creator | Jessberger, Rolf | es |
dc.creator | Chacón Rodríguez, Mariola | es |
dc.date.accessioned | 2022-10-25T18:04:00Z | |
dc.date.available | 2022-10-25T18:04:00Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Nabi, D., Drechsler, H., Pschirer, J., Korn, F., Schuler, N., Diez, S.,...,Chacón Rodríguez, M. (2021). CENP-V is required for proper chromosome segregation through interaction with spindle microtubules in mouse oocytes. Nature Communications, 12 (1), 6547. https://doi.org/10.1038/s41467-021-26826-3. | |
dc.identifier.issn | 2041-1723 | es |
dc.identifier.uri | https://hdl.handle.net/11441/138324 | |
dc.description.abstract | Proper chromosome segregation is essential to avoid aneuploidy, yet this process fails with increasing age in mammalian oocytes. Here we report a role for the scarcely described protein CENP-V in oocyte spindle formation and chromosome segregation. We show that depending on the oocyte maturation state, CENP-V localizes to centromeres, to microtubule organizing centers, and to spindle microtubules. We find that Cenp-V−/− oocytes feature severe deficiencies, including metaphase I arrest, strongly reduced polar body extrusion, increased numbers of mis-aligned chromosomes and aneuploidy, multipolar spindles, unfocused spindle poles and loss of kinetochore spindle fibres. We also show that CENP-V protein binds, diffuses along, and bundles microtubules in vitro. The spindle assembly checkpoint arrests about half of metaphase I Cenp-V−/− oocytes from young adults only. This finding suggests checkpoint weakening in ageing oocytes, which mature despite carrying mis-aligned chromosomes. Thus, CENP-V is a microtubule bundling protein crucial to faithful oocyte meiosis, and Cenp-V−/− oocytes reveal age-dependent weakening of the spindle assembly checkpoint. | es |
dc.description.sponsorship | Sociedad Alemana de Investigación DFG, JE150/25-1, 417890911 | es |
dc.format | application/pdf | es |
dc.format.extent | 16 p. | es |
dc.language.iso | eng | es |
dc.publisher | Springer Nature | es |
dc.relation.ispartof | Nature Communications, 12 (1), 6547. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Animals | es |
dc.subject | Chromosome Segregation | es |
dc.subject | Female | es |
dc.subject | M Phase Cell Cycle Checkpoints | es |
dc.subject | Mammalia | es |
dc.subject | centrome protein v | es |
dc.subject | chromosome | es |
dc.subject | amino terminal sequence | es |
dc.title | CENP-V is required for proper chromosome segregation through interaction with spindle microtubules in mouse oocytes | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Biología Celular | es |
dc.relation.projectID | DFG, JE150/25-1 | es |
dc.relation.projectID | 417890911 | es |
dc.relation.publisherversion | https://doi.org/10.1038/s41467-021-26826-3 | es |
dc.identifier.doi | 10.1038/s41467-021-26826-3 | es |
dc.journaltitle | Nature Communications | es |
dc.publication.volumen | 12 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | 6547 | es |
dc.contributor.funder | Sociedad Alemana de Investigación | es |