dc.creator | Gallardo, Mercedes | es |
dc.creator | Luna Varo, Rosa María | es |
dc.creator | Erdjument-Bromage, Hediye | es |
dc.creator | Tempst, Paul | es |
dc.creator | Aguilera López, Andrés | es |
dc.date.accessioned | 2022-09-23T07:39:36Z | |
dc.date.available | 2022-09-23T07:39:36Z | |
dc.date.issued | 2003 | |
dc.identifier.citation | Gallardo, M., Luna Varo, R.M., Erdjument-Bromage, H., Tempst, P. y Aguilera López, A. (2003). Nab2p and the Thp1p-Sac3p Complex Functionally Interact at the Interface between Transcription and mRNA Metabolism. Journal of Biological Chemistry, 278 (26), 24225-24232. | |
dc.identifier.issn | 1083-351X | es |
dc.identifier.uri | https://hdl.handle.net/11441/137321 | |
dc.description.abstract | THP1 is a conserved eukaryotic gene whose null mutations confer, in yeast, transcription and genetic instability phenotypes and RNA export defects similar to those of the THO/TREX complex null mutations. In a search for multicopy suppressors of the transcription defect of thp1Δ cells, we identified the poly(A)+ RNA-binding heterogeneous nuclear ribonucleoprotein Nab2p. Multicopy NAB2 also suppressed the RNA export defect of thp1Δ cells. This result suggests a functional relationship between Thp1p and Nab2p. Consistently, the leaky mutation nab2-1 conferred a transcription defect and hyper-recombination phenotype similar to those of thp1Δ, although to a minor degree. Reciprocally, a purified His6-tagged Thp1p fusion bound RNA in vitro. In a different approach, we show by Western analyses that a highly purified Thp1p-Sac3p complex does not contain components of THO/TREX and that sac3Δ confers a transcription defect and hyper-recombination phenotype identical to those of thp1Δ. mRNA degradation was not affected in thp1Δ mutants, implying that their expression defects are not due to mRNA decay. This indicates that Thp1p-Sac3p is a structural and functional unit. Altogether, our results suggest that Thp1p-Sac3p and Nab2p are functionally related heterogeneous nuclear ribonucleoproteins that define a further link between mRNA metabolism and transcription. | es |
dc.format | application/pdf | es |
dc.format.extent | 8 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Journal of Biological Chemistry, 278 (26), 24225-24232. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Nab2p and the Thp1p-Sac3p Complex Functionally Interact at the Interface between Transcription and mRNA Metabolism | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | https://dx.doi.org/10.1074/jbc.M302900200 | es |
dc.identifier.doi | 10.1074/jbc.M302900200 | es |
dc.journaltitle | Journal of Biological Chemistry | es |
dc.publication.volumen | 278 | es |
dc.publication.issue | 26 | es |
dc.publication.initialPage | 24225 | es |
dc.publication.endPage | 24232 | es |