Article
Circulating tumor cells enumeration from the portal vein for risk stratification in early pancreatic cancer patients
Author/s | Padillo Ruiz, Francisco Javier
Suarez Artacho, Gonzalo Pereira Arenas, Sheila Calero-Castro, Francisco José Tinoco, José Marín Gómez, Luis Miguel Bernal, Carmen Cepeda Franco, Carmen Álamo Martínez, José María Almoguera, Francisco Macher, Hada C. Villanueva, Paula García-Fernández, Francisco-José Gallego, Inmaculada Gómez Bravo, Miguel Ángel Denninghoff, Valeria Serrano, María José |
Department | Universidad de Sevilla. Departamento de Cirugía Universidad de Sevilla. Departamento de Medicina |
Publication Date | 2021-12-07 |
Deposit Date | 2022-09-21 |
Published in |
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Abstract | Background: Effective biomarkers are needed to enable personalized medicine for pancreatic cancer patients. This study analyzes the prognostic value, in early pancreatic cancer, of single circulating tumor cell (CTC) and ... Background: Effective biomarkers are needed to enable personalized medicine for pancreatic cancer patients. This study analyzes the prognostic value, in early pancreatic cancer, of single circulating tumor cell (CTC) and CTC clusters from the central venous catheter (CVC) and portal blood (PV). Methods: In total, 7 mL of PV and CVC blood from 35 patients with early pancreatic cancer were analyzed. CTC were isolated using a positive immunomagnetic selection. The detection and identification of CTC were performed by immunocytochemistry (ICC) and were analyzed by Epi-fluorescence and confocal microscopy. Results: CTC and the clusters were detected both in PV and CVC. In both samples, the CTC number per cluster was higher in patients with grade three or poorly differentiated tumors (G3) than in patients with well (G1) or moderately (G2) differentiated. Patients with fewer than 185 CTC in PV exhibited a longer OS than patients with more than 185 CTC (24.5 vs. 10.0 months; p = 0.018). Similarly, patients with fewer than 15 clusters in PV showed a longer OS than patients with more than 15 clusters (19 vs. 10 months; p = 0.004). These significant correlations were not observed in CVC analyses. Conclusions: CTC presence in PV could be an important prognostic factor to predict poor prognosis in early pancreatic cancer. In addition, the number of clustered-CTC correlate to a tumor negative differentiation degree and, therefore, could be used as a diagnostic biomarker for pancreatic cancer. |
Project ID. | PI16/01465
PI19/01821 |
Citation | Padillo Ruiz, F.J., Suarez Artacho, G., Pereira Arenas, S., Calero-Castro, F.J., Tinoco, J., Marín Gómez, L.M.,...,Serrano, M.J. (2021). Circulating tumor cells enumeration from the portal vein for risk stratification in early pancreatic cancer patients. Cancers, 13 (24) |
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