dc.creator | Cañas, Juan Carlos | es |
dc.creator | García Rubio, María Luisa | es |
dc.creator | García, Alicia | es |
dc.creator | Antequera, Francisco | es |
dc.creator | Gómez González, Belén | es |
dc.creator | Aguilera López, Andrés | es |
dc.date.accessioned | 2022-09-16T14:31:25Z | |
dc.date.available | 2022-09-16T14:31:25Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Cañas, J.C., García Rubio, M.L., García, A., Antequera, F., Gómez González, B. y Aguilera López, A. (2022). A role for the Saccharomyces cerevisiae Rtt109 histone acetyltransferase in R-loop homeostasis and associated genome instability. Genetics, 222 (1), iyac108. | |
dc.identifier.issn | 0016-6731 | es |
dc.identifier.issn | 1943-2631 | es |
dc.identifier.uri | https://hdl.handle.net/11441/137151 | |
dc.description.abstract | The stability of the genome is occasionally challenged by the formation of DNA-RNA hybrids and R-loops, which can be influenced by the chromatin context. This is mainly due to the fact that DNA-RNA hybrids hamper the progression of replication forks, leading to fork stalling and, ultimately, DNA breaks. Through a specific screening of chromatin modifiers performed in the yeast Saccharomyces cerevisiae, we have found that the Rtt109 histone acetyltransferase is involved in several steps of R-loop-metabolism and their associated genetic instability. On the one hand, Rtt109 prevents DNA-RNA hybridization by the acetylation of histone H3 lysines 14 and 23 and, on the other hand, it is involved in the repair of replication-born DNA breaks, such as those that can be caused by R-loops, by acetylating lysines 14 and 56. In addition, Rtt109 loss renders cells highly sensitive to replication stress in combination with R-loop-accumulating THO-complex mutants. Our data evidence that the chromatin context simultaneously influences the occurrence of DNA-RNA hybrid-associated DNA damage and its repair, adding complexity to the source of R-loop-associated genetic instability. | es |
dc.description.sponsorship | European Research Council ERC2014 AdG669898 TARLOOP | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad BFU2016-75058-P | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación PID2019-104270GB-I00, PID2020-118423GB-I00 | es |
dc.description.sponsorship | European Union US-1258654 | es |
dc.description.sponsorship | Junta de Andalucía P12-BIO-1238 | es |
dc.format | application/pdf | es |
dc.format.extent | 12 p. | es |
dc.language.iso | eng | es |
dc.publisher | Genetics Society of America | es |
dc.relation.ispartof | Genetics, 222 (1), iyac108. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | DNA–RNA hybrids | es |
dc.subject | Genetic instability | es |
dc.subject | Histone acetylation | es |
dc.subject | R-loops | es |
dc.subject | Sister-chromatid recombination | es |
dc.title | A role for the Saccharomyces cerevisiae Rtt109 histone acetyltransferase in R-loop homeostasis and associated genome instability | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | ERC2014 AdG669898 TARLOOP | es |
dc.relation.projectID | BFU2016-75058-P | es |
dc.relation.projectID | PID2019-104270GB-I00 | es |
dc.relation.projectID | PID2020-118423GB-I00 | es |
dc.relation.projectID | US-1258654 | es |
dc.relation.projectID | P12-BIO-1238 | es |
dc.relation.publisherversion | https://doi.org/10.1093/genetics/iyac108 | es |
dc.identifier.doi | 10.1093/genetics/iyac108 | es |
dc.journaltitle | Genetics | es |
dc.publication.volumen | 222 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | iyac108 | es |
dc.contributor.funder | European Research Council (ERC) | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | es |
dc.contributor.funder | European Union (UE) | es |
dc.contributor.funder | Junta de Andalucía | es |