dc.creator | Santigosa, Elia | es |
dc.creator | Pedersen-Bjergaard, Stig | es |
dc.creator | Giménez Gómez, Pablo | es |
dc.creator | Muñoz, María | es |
dc.creator | Ramos Payán, María Dolores | es |
dc.date.accessioned | 2022-05-16T13:24:16Z | |
dc.date.available | 2022-05-16T13:24:16Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Santigosa, E., Pedersen-Bjergaard, S., Giménez Gómez, P., Muñoz, M. y Ramos Payán, M.D. (2022). A rapid and versatile microfluidic method for the simultaneous extraction of polar and non-polar basic pharmaceuticals from human urine. Analytica Chimica Acta, 1208, 339829. | |
dc.identifier.issn | 0003-2670 | es |
dc.identifier.issn | 1873-4324 | es |
dc.identifier.uri | https://hdl.handle.net/11441/133343 | |
dc.description.abstract | In sample preparation, simultaneous extraction of analytes of very different polarity from biological matrixes represents a challenge. In this work, verapamil hydrochloride (VRP), amitriptyline (AMP), tyramine (TYR), atenolol (ATN), metopropol (MTP) and nortriptyline (NRP) were used as basic model analytes and simultaneously extracted from urine samples by liquid-phase microextraction (LPME) in a microfluidic device. The model analytes (target compounds) were pharmaceuticals with 0.4 < log P < 5. Different organic solvents and mixtures of them were investigated as supported liquid membrane (SLM), and a mixture of 2:1 (v/v) tributyl phosphate (TBP) and dihexyl ether (DHE) was found to be highly efficient for the simultaneous extraction of the non-polar and polar model analytes. TBP reduced the intrinsic hydrophobicity of the SLM and facilitated extraction of polar analytes, while DHE served to minimize trapping of non-polar analytes. Sample and acceptor phase composition were adjusted to pH 12 and pH 1.5, respectively. Urine samples were pumped into the microfluidic system at 1 μL min-1 and the extraction was completed in 7 min. Recoveries exceeded 78% for the target analytes, and the relative standard deviation (n = 4) was below 7% in all cases. Using five microliters of SLM, the microfluidic extraction system showed good long-term stability, and the same SLM was used for more than 18 consecutive extractions. | es |
dc.description.sponsorship | Agencia de Gestió d'Ajusts Universitaris i the Recerca 2017-SGR-329 | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación PGC2018-096608-B-C21 | es |
dc.format | application/pdf | es |
dc.format.extent | 9 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Analytica Chimica Acta, 1208, 339829. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Basic drugs | es |
dc.subject | Liquid phase microextraction | es |
dc.subject | Microfluidic | es |
dc.subject | Supported liquid membrane | es |
dc.subject | Urine samples | es |
dc.title | A rapid and versatile microfluidic method for the simultaneous extraction of polar and non-polar basic pharmaceuticals from human urine | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Química Analítica | es |
dc.relation.projectID | 2017-SGR-329 | es |
dc.relation.projectID | PGC2018-096608-B-C21 | es |
dc.relation.publisherversion | https://doi.org/10.1016/j.aca.2022.339829 | es |
dc.identifier.doi | 10.1016/j.aca.2022.339829 | es |
dc.journaltitle | Analytica Chimica Acta | es |
dc.publication.volumen | 1208 | es |
dc.publication.initialPage | 339829 | es |
dc.contributor.funder | Agencia de Gestió d'Ajusts Universitaris i the Recerca | es |
dc.contributor.funder | Agencia Estatal de Investigación. España | es |
dc.description.awardwinning | Premio Mensual Publicación Científica Destacada de la US. Facultad de Química | |