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A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines

dc.creatorAmador Álvarez, Aídaes
dc.creatorRodríguez Prieto, Ismaeles
dc.creatorPardal Redondo, Ricardoes
dc.creatorVega Moreno, Francisco Manueles
dc.creatorGómez Muñoz, María Ángeleses
dc.date.accessioned2022-03-28T07:37:35Z
dc.date.available2022-03-28T07:37:35Z
dc.date.issued2022
dc.identifier.citationAmador Álvarez, A., Rodríguez Prieto, I., Pardal Redondo, R., Vega Moreno, F.M. y Gómez Muñoz, M.Á. (2022). A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines. STAR Protocols, 3 (2), 101260.
dc.identifier.issn2666-1667es
dc.identifier.urihttps://hdl.handle.net/11441/131318
dc.description.abstractThe existence of a subpopulation of undifferentiated cells with stem-like properties has been suggested in neuroblastoma tumors, but a definitive biomarker for their successful isolation is missing. Here we describe an in vitro culture system for the enrichment in undifferentiated stem-like tumor cells for subsequent functional assays. We make use of clonal non-adherent cell culture conditions together with cell sorting with specific expression markers. This protocol allows for the differential study of heterogeneous cell population in neuroblastoma tumors. For complete details on the use and execution of this protocol, please refer to Vega et al. (2019).es
dc.formatapplication/pdfes
dc.format.extent16 p.es
dc.language.isoenges
dc.publisherCell Presses
dc.relation.ispartofSTAR Protocols, 3 (2), 101260.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCell Biologyes
dc.subjectCell culturees
dc.subjectCell isolationes
dc.subjectSingle Celles
dc.subjectFlow Cytometry/Mass Cytometryes
dc.subjectCanceres
dc.subjectStem Cellses
dc.titleA protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lineses
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Biología Celulares
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Fisiología Médica y Biofísicaes
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.xpro.2022.101260es
dc.identifier.doi10.1016/j.xpro.2022.101260es
dc.journaltitleSTAR Protocolses
dc.publication.volumen3es
dc.publication.issue2es
dc.publication.endPage101260es

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