Artículo
A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines
Autor/es | Amador Álvarez, Aída
Rodríguez Prieto, Ismael Pardal Redondo, Ricardo Vega Moreno, Francisco Manuel Gómez Muñoz, María Ángeles |
Departamento | Universidad de Sevilla. Departamento de Biología Celular Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica |
Fecha de publicación | 2022 |
Fecha de depósito | 2022-03-28 |
Publicado en |
|
Resumen | The existence of a subpopulation of undifferentiated cells with stem-like properties has been suggested in neuroblastoma tumors, but a definitive biomarker for their successful isolation is missing. Here we describe an in ... The existence of a subpopulation of undifferentiated cells with stem-like properties has been suggested in neuroblastoma tumors, but a definitive biomarker for their successful isolation is missing. Here we describe an in vitro culture system for the enrichment in undifferentiated stem-like tumor cells for subsequent functional assays. We make use of clonal non-adherent cell culture conditions together with cell sorting with specific expression markers. This protocol allows for the differential study of heterogeneous cell population in neuroblastoma tumors. For complete details on the use and execution of this protocol, please refer to Vega et al. (2019). |
Cita | Amador Álvarez, A., Rodríguez Prieto, I., Pardal Redondo, R., Vega Moreno, F.M. y Gómez Muñoz, M.Á. (2022). A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines. STAR Protocols, 3 (2), 101260. |
Ficheros | Tamaño | Formato | Ver | Descripción |
---|---|---|---|---|
pubmain.pdf | 2.308Mb | [PDF] | Ver/ | |