dc.creator | Pontel, Lucas B. | es |
dc.creator | Langenick, Judith | es |
dc.creator | Valle Rosado, Iván | es |
dc.creator | Zhang, Xiao-Yin | es |
dc.creator | Traynor, David | es |
dc.creator | Kay, Robert R. | es |
dc.creator | Patel, Ketan J. | es |
dc.date.accessioned | 2022-03-22T10:52:19Z | |
dc.date.available | 2022-03-22T10:52:19Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Pontel, L.B., Langenick, J., Valle Rosado, I., Zhang, X., Traynor, ., Kay, .R. y Patel, K.J. (2016). Xpf suppresses the mutagenic consequences of phagocytosis in Dictyostelium. Journal of Cell Science, 129 (24), 4449-4454. | |
dc.identifier.issn | 0021-9533 | es |
dc.identifier.issn | 1477-9137 | es |
dc.identifier.uri | https://hdl.handle.net/11441/131131 | |
dc.description.abstract | As time passes, mutations accumulate in the genomes of all living
organisms. These changes promote genetic diversity, but also
precipitate ageing and the initiation of cancer. Food is a common
source of mutagens, but little is known about how nutritional factors
cause lasting genetic changes in the consuming organism. Here, we
describe an unusual genetic interaction between DNA repair in the
unicellular amoeba Dictyostelium discoideum and its natural bacterial
food source. We found that Dictyostelium deficient in the DNA repair
nuclease Xpf (xpf−) display a severe and specific growth defect when
feeding on bacteria. Despite being proficient in the phagocytosis and
digestion of bacteria, over time, xpf− Dictyostelium feeding on bacteria
cease to grow and in many instances die. The Xpf nuclease activity
is required for sustained growth using a bacterial food source.
Furthermore, the ingestion of this food source leads to a striking
accumulation of mutations in the genome of xpf− Dictyostelium. This
work therefore establishes Dictyostelium as a model genetic system to
dissect nutritional genotoxicity, providing insight into how phagocytosis
can induce mutagenesis and compromise survival fitness. | es |
dc.description.sponsorship | Medical Research Council (MRC) de Reino Unido. MC_U105178811 y MC_U105115237 | es |
dc.description.sponsorship | Wellcome Trust de Reino Unido. WT106202 | es |
dc.format | application/pdf | es |
dc.format.extent | 6 p. | es |
dc.language.iso | eng | es |
dc.publisher | The Company of Biologists | es |
dc.relation.ispartof | Journal of Cell Science, 129 (24), 4449-4454. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Xpf | es |
dc.subject | Phagocytosis | es |
dc.subject | Mutagenesis | es |
dc.subject | Dictyostelium | es |
dc.subject | DNA repair | es |
dc.title | Xpf suppresses the mutagenic consequences of phagocytosis in Dictyostelium | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | MC_U105178811 | es |
dc.relation.projectID | MC_U105115237 | es |
dc.relation.projectID | WT106202 | es |
dc.relation.publisherversion | https://doi.org/10.1242/jcs.196337 | es |
dc.identifier.doi | 10.1242/jcs.196337 | es |
dc.journaltitle | Journal of Cell Science | es |
dc.publication.volumen | 129 | es |
dc.publication.issue | 24 | es |
dc.publication.initialPage | 4449 | es |
dc.publication.endPage | 4454 | es |
dc.contributor.funder | Medical Research Council (MRC). UK | es |
dc.contributor.funder | Wellcome Trust. UK | es |
dc.contributor.funder | Cancer Research. UK | es |