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dc.creatorUrdaneta Páez, Verónica Carolinaes
dc.creatorCasadesús Pursals, Josepes
dc.date.accessioned2022-03-21T12:42:40Z
dc.date.available2022-03-21T12:42:40Z
dc.date.issued2018
dc.identifier.citationUrdaneta Páez, V.C. y Casadesús Pursals, J. (2018). Adaptation of Salmonella enterica to bile: essential role of AcrAB-mediated efflux. Environmental Microbiology, 20 (4), 1405-1418.
dc.identifier.issn1462-2912es
dc.identifier.issn1462-2920es
dc.identifier.urihttps://hdl.handle.net/11441/131094
dc.description.abstractAdaptation to bile is the ability to endure the lethal effects of bile salts after growth on sublethal concentrations. Surveys of adaptation to bile in Salmonella enterica ser. Tyhimurium reveal that active efflux is essential for adaptation while other bacterial functions involved in bile resistance are not. Among S. enterica mutants lacking one or more efflux systems, only strains lacking AcrAB are unable to adapt, thus revealing an essential role for AcrAB. Transcription of the acrAB operon is upregulated in the presence of a sublethal concentration of sodium deoxycholate (DOC) while other efflux loci are either weakly upregulated or irresponsive. Upregulation of acrAB transcription is strong during exponential growth, and weak in stationary cultures. Single cell analysis of ethidium bromide accumulation indicates that DOC-induced AcrAB-mediated efflux occurs in both exponential and stationary cultures. Upregulation of acrAB expression may thus be crucial at early stages of adaptation, while sustained AcrAB activity may be sufficient to confer bile resistance in nondividing cells.es
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO) de España y el Fondo Regional Europeo (FEDER) BIO2013–44220-R y BIO2016–75235-Pes
dc.description.sponsorshipConsejería de Innovación, Ciencia y Empresa, Junta de Andalucía. CVI-5879es
dc.formatapplication/pdfes
dc.format.extent14 p.es
dc.language.isoenges
dc.publisherSociety for Applied Microbiologyes
dc.relation.ispartofEnvironmental Microbiology, 20 (4), 1405-1418.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleAdaptation of Salmonella enterica to bile: essential role of AcrAB-mediated effluxes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.projectIDBIO2013–44220-Res
dc.relation.projectIDBIO2016–75235-Pes
dc.relation.projectIDCVI-5879es
dc.relation.publisherversionhttps://doi.org/10.1111/1462-2920.14047es
dc.identifier.doi10.1111/1462-2920.14047es
dc.journaltitleEnvironmental Microbiologyes
dc.publication.volumen20es
dc.publication.issue4es
dc.publication.initialPage1405es
dc.publication.endPage1418es
dc.contributor.funderMinisterio de Economía y Competitividad (MINECO). Españaes
dc.contributor.funderEuropean Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)es
dc.contributor.funderJunta de Andalucíaes

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