Mostrar el registro sencillo del ítem

Artículo

dc.creatorTovar Méndez, Alejandroes
dc.creatorMatamoros, Manuel A.es
dc.creatorBustos Sanmamed, Pilares
dc.creatorDietz, Karl Josefes
dc.creatorCejudo Fernández, Francisco Javieres
dc.creatorRouhier, Nicolases
dc.creatorSato, Shuseies
dc.creatorTabata, Satoshies
dc.creatorBecana, Manueles
dc.date.accessioned2022-03-16T09:38:43Z
dc.date.available2022-03-16T09:38:43Z
dc.date.issued2011
dc.identifier.citationTovar Méndez, A., Matamoros, M.A., Bustos Sanmamed, P., Dietz, K.J., Cejudo Fernández, F.J., Rouhier, N.,...,Becana, M. (2011). Peroxiredoxins and NADPH-Dependent Thioredoxin Systems in the Model Legume Lotus japonicus. Plant Physiology, 156 (3), 1535-1547.
dc.identifier.issn1532-2548es
dc.identifier.issn0032-0889es
dc.identifier.urihttps://hdl.handle.net/11441/130859
dc.description.abstractPeroxiredoxins (Prxs), thioredoxins (Trxs), and NADPH-thioredoxin reductases (NTRs) constitute central elements of the thiol-disulfide redox regulatory network of plant cells. This study provides a comprehensive survey of this network in the model legume Lotus japonicus. The aims were to identify and characterize these gene families and to assess whether the NTR-Trx systems are operative in nodules. Quantitative reverse transcription-polymerase chain reaction and immunological and proteomic approaches were used for expression profiling. We identified seven Prx, 14 Trx, and three NTR functional genes. The PrxQ1 gene was found to be transcribed in two alternative spliced variants and to be expressed at high levels in leaves, stems, petals, pods, and seeds and at low levels in roots and nodules. The 1CPrx gene showed very high expression in the seed embryos and low expression in vegetative tissues and was induced by nitric oxide and cytokinins. In sharp contrast, cytokinins down-regulated all other Prx genes, except PrxQ1, in roots and nodules, but only 2CPrxA and PrxQ1 in leaves. Gene-specific changes in Prx expression were also observed in response to ethylene, abscisic acid, and auxins. Nodules contain significant mRNA and protein amounts of cytosolic PrxIIB, Trxh1, and NTRA and of plastidic NTRC. Likewise, they express cytosolic Trxh3, Trxh4, Trxh8, and Trxh9, mitochondrial PrxIIF and Trxo, and plastidic Trxm2, Trxm4, and ferredoxin-Trx reductase. These findings reveal a complex regulation of Prxs that is dependent on the isoform, tissue, and signaling molecule and support that redox NTR-Trx systems are functional in the cytosol, mitochondria, and plastids of nodules.es
dc.formatapplication/pdfes
dc.format.extent13 p.es
dc.language.isoenges
dc.publisherAmerican Society of Plant Biologistses
dc.relation.ispartofPlant Physiology, 156 (3), 1535-1547.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titlePeroxiredoxins and NADPH-Dependent Thioredoxin Systems in the Model Legume Lotus japonicuses
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.publisherversionhttps://doi.org/10.1104/pp.111.177196es
dc.identifier.doi10.1104/pp.111.177196es
dc.journaltitlePlant Physiologyes
dc.publication.volumen156es
dc.publication.issue3es
dc.publication.initialPage1535es
dc.publication.endPage1547es
dc.identifier.sisius20191121es

FicherosTamañoFormatoVerDescripción
plphys_v156_3_1535.pdf778.7KbIcon   [PDF] Ver/Abrir  

Este registro aparece en las siguientes colecciones

Mostrar el registro sencillo del ítem

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Excepto si se señala otra cosa, la licencia del ítem se describe como: Attribution-NonCommercial-NoDerivatives 4.0 Internacional