dc.creator | Depuydt, Julie | es |
dc.creator | Baeyens, Ans | es |
dc.creator | Barnard, Stephen | es |
dc.creator | Beinke, Christina | es |
dc.creator | Benedek, Anett | es |
dc.creator | Beukes, Philip | es |
dc.creator | Domínguez García, Inmaculada | es |
dc.creator | Pastor Carrillo, Nuria María | es |
dc.creator | Vral, Anne | es |
dc.date.accessioned | 2022-03-11T13:08:10Z | |
dc.date.available | 2022-03-11T13:08:10Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Depuydt, J., Baeyens, A., Barnard, S., Beinke, C., Benedek, A., Beukes, P.,...,Vral, A. (2016). RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay). International Journal of Radiation Biology, 93 (1), 36-47. | |
dc.identifier.issn | 0955-3002 | es |
dc.identifier.issn | 1362-3095 | es |
dc.identifier.uri | https://hdl.handle.net/11441/130709 | |
dc.description.abstract | Purpose: In the framework of the ‘Realizing the European Network of Biodosimetry’ (RENEB) project, two intercomparison exercises were conducted to assess the suitability of an optimized version of the cytokinesis-block micronucleus assay, and to evaluate the capacity of a large laboratory network performing biodosimetry for radiation emergency triages. Twelve European institutions participated in the first exercise, and four non-RENEB labs were added in the second one.
Materials and methods: Irradiated blood samples were shipped to participating labs, whose task was to culture these samples and provide a blind dose estimate. Micronucleus analysis was performed by automated, semi-automated and manual procedures.
Results: The dose estimates provided by network laboratories were in good agreement with true administered doses. The most accurate estimates were reported for low dose points (≤ 0.94 Gy). For higher dose points (≥ 2.7 Gy) a larger variation in estimates was observed, though in the second exercise the number of acceptable estimates increased satisfactorily. Higher accuracy was achieved with the semi-automated method.
Conclusion: The results of the two exercises performed by our network demonstrate that the micronucleus assay is a useful tool for large-scale radiation emergencies, and can be successfully implemented within a large network of laboratories. | es |
dc.description.sponsorship | VII Programa Marco de Investigación y Desarrollo (VIIPM) de la Unión Europea. nº 295513 | es |
dc.description.sponsorship | University Development Cooperation "VLIR Own Initiative Programme" enetre Bélgica and Sudáfrica. ZEIN2011PR387 | es |
dc.format | application/pdf | es |
dc.format.extent | 14 p. | es |
dc.language.iso | eng | es |
dc.publisher | Taylor & Francis LTD | es |
dc.relation.ispartof | International Journal of Radiation Biology, 93 (1), 36-47. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Biodosimetry | es |
dc.subject | micronucleus assay | es |
dc.subject | triage | es |
dc.subject | radiation accident | es |
dc.subject | RENEB | es |
dc.title | RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay) | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Biología Celular | es |
dc.relation.projectID | VIIPM 295513 | es |
dc.relation.projectID | ZEIN2011PR387 | es |
dc.relation.publisherversion | https://doi.org/10.1080/09553002.2016.1206231 | es |
dc.identifier.doi | 10.1080/09553002.2016.1206231 | es |
dc.journaltitle | International Journal of Radiation Biology | es |
dc.publication.volumen | 93 | es |
dc.publication.issue | 1 | es |
dc.publication.initialPage | 36 | es |
dc.publication.endPage | 47 | es |
dc.identifier.sisius | 21293936 | es |
dc.contributor.funder | European Union | es |