Artículo
RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay)
Autor/es | Depuydt, Julie
Baeyens, Ans Barnard, Stephen Beinke, Christina Benedek, Anett Beukes, Philip Domínguez García, Inmaculada Pastor Carrillo, Nuria María Vral, Anne |
Departamento | Universidad de Sevilla. Departamento de Biología Celular |
Fecha de publicación | 2016 |
Fecha de depósito | 2022-03-11 |
Publicado en |
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Resumen | Purpose: In the framework of the ‘Realizing the European Network of Biodosimetry’ (RENEB) project, two intercomparison exercises were conducted to assess the suitability of an optimized version of the cytokinesis-block ... Purpose: In the framework of the ‘Realizing the European Network of Biodosimetry’ (RENEB) project, two intercomparison exercises were conducted to assess the suitability of an optimized version of the cytokinesis-block micronucleus assay, and to evaluate the capacity of a large laboratory network performing biodosimetry for radiation emergency triages. Twelve European institutions participated in the first exercise, and four non-RENEB labs were added in the second one. Materials and methods: Irradiated blood samples were shipped to participating labs, whose task was to culture these samples and provide a blind dose estimate. Micronucleus analysis was performed by automated, semi-automated and manual procedures. Results: The dose estimates provided by network laboratories were in good agreement with true administered doses. The most accurate estimates were reported for low dose points (≤ 0.94 Gy). For higher dose points (≥ 2.7 Gy) a larger variation in estimates was observed, though in the second exercise the number of acceptable estimates increased satisfactorily. Higher accuracy was achieved with the semi-automated method. Conclusion: The results of the two exercises performed by our network demonstrate that the micronucleus assay is a useful tool for large-scale radiation emergencies, and can be successfully implemented within a large network of laboratories. |
Agencias financiadoras | European Union |
Identificador del proyecto | VIIPM 295513
ZEIN2011PR387 |
Cita | Depuydt, J., Baeyens, A., Barnard, S., Beinke, C., Benedek, A., Beukes, P.,...,Vral, A. (2016). RENEB intercomparison exercises analyzing micronuclei (Cytokinesis-block Micronucleus Assay). International Journal of Radiation Biology, 93 (1), 36-47. |
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