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dc.creatorBegley, Victoria Sarahes
dc.creatorJordán Pla, Antonioes
dc.creatorPeñate Salas, Xeniaes
dc.creatorGarrido Godino, Ana Isabeles
dc.creatorChallal, Dricees
dc.creatorCuevas Bermúdez, Abeles
dc.creatorMitjavila, Adriàes
dc.creatorBarucco, Maraes
dc.creatorGutiérrez Pozo, Gabrieles
dc.creatorSingh, Abhyudaies
dc.creatorAlepuz, Paula M.es
dc.creatorChávez de Diego, Sebastiánes
dc.date.accessioned2021-09-30T15:11:22Z
dc.date.available2021-09-30T15:11:22Z
dc.date.issued2021
dc.identifier.citationBegley, V.S., Jordán Pla, A., Peñate Salas, X., Garrido Godino, A.I., Challal, D., Cuevas Bermúdez, A.,...,Chávez de Diego, S. (2021). Xrn1 influence on gene transcription results from the combination of general effects on elongating RNA pol II and gene-specific chromatin configuration. RNA Biology, 18 (9), 1310-1323.
dc.identifier.issn1547-6286es
dc.identifier.issn1555-8584es
dc.identifier.urihttps://hdl.handle.net/11441/126390
dc.description.abstractmRNA homoeostasis is favoured by crosstalk between transcription and degradation machineries. Both the Ccr4-Not and the Xrn1-decaysome complexes have been described to influence transcription. While Ccr4-Not has been shown to directly stimulate transcription elongation, the information available on how Xrn1 influences transcription is scarce and contradictory. In this study we have addressed this issue by mapping RNA polymerase II (RNA pol II) at high resolution, using CRAC and BioGRO-seq techniques in Saccharomyces cerevisiae. We found significant effects of Xrn1 perturbation on RNA pol II profiles across the genome. RNA pol II profiles at 5ʹ exhibited significant alterations that were compatible with decreased elongation rates in the absence of Xrn1. Nucleosome mapping detected altered chromatin configuration in the gene bodies. We also detected accumulation of RNA pol II shortly upstream of polyadenylation sites by CRAC, although not by BioGRO-seq, suggesting higher frequency of backtracking before pre-mRNA cleavage. This phenomenon was particularly linked to genes with poorly positioned nucleosomes at this position. Accumulation of RNA pol II at 3ʹ was also detected in other mRNA decay mutants. According to these and other pieces of evidence, Xrn1 seems to influence transcription elongation at least in two ways: by directly favouring elongation rates and by a more general mechanism that connects mRNA decay to late elongation.es
dc.description.sponsorshipMinisterio de Economía y Competitividad BFU2016-77728- C3-1-P, BFU2016-77728-C3-3-P, BFU2016- 77728-C3-2-P, RED2018-102467-Tes
dc.description.sponsorshipJunta de Andalucía BIO271, US-1256285, BIO258, UJA 1260360es
dc.description.sponsorshipGeneralitat Valenciana AICO/2019/088es
dc.formatapplication/pdfes
dc.format.extent14 p.es
dc.language.isoenges
dc.publisherTaylor & Francises
dc.relation.ispartofRNA Biology, 18 (9), 1310-1323.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject3ʹ pre-mRNA processinges
dc.subjectmRNA bufferinges
dc.subjectmRNA decayes
dc.subjectmRNA decay/gene transcription crosstalkes
dc.subjectTranscription elongationes
dc.subjectXrn1es
dc.titleXrn1 influence on gene transcription results from the combination of general effects on elongating RNA pol II and gene-specific chromatin configurationes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.projectIDBFU2016-77728- C3-1-Pes
dc.relation.projectIDBFU2016-77728-C3-3-Pes
dc.relation.projectIDBFU2016- 77728-C3-2-Pes
dc.relation.projectIDRED2018-102467-Tes
dc.relation.projectIDBIO271es
dc.relation.projectIDUS-1256285es
dc.relation.projectIDBIO271es
dc.relation.projectIDUS-1256285es
dc.relation.projectIDBIO258es
dc.relation.projectIDUJA 1260360es
dc.relation.projectIDAICO/2019/088es
dc.relation.publisherversionhttps://doi.org/10.1080/15476286.2020.1845504es
dc.identifier.doi10.1080/15476286.2020.1845504es
dc.journaltitleRNA Biologyes
dc.publication.volumen18es
dc.publication.issue9es
dc.publication.initialPage1310es
dc.publication.endPage1323es

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