dc.creator | Gutiérrez Beltrán, Emilio | es |
dc.creator | Elander, Pernilla H. | es |
dc.creator | Dalman, Kerstin | es |
dc.creator | Dayhoff, Guy W. II | es |
dc.creator | Moschou, Panagiotis N. | es |
dc.creator | Uversky, Vladimir N. | es |
dc.creator | Crespo, José L. | es |
dc.creator | Bozhkov, Peter V. | es |
dc.date.accessioned | 2021-09-07T14:05:10Z | |
dc.date.available | 2021-09-07T14:05:10Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Gutiérrez Beltrán, E., Elander, P.H., Dalman, K., Dayhoff, G.W.I., Moschou, P.N., Uversky, V.N.,...,Bozhkov, P.V. (2021). Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis. EMBO Journal, 40 (17), e105043. | |
dc.identifier.issn | 0261-4189 | es |
dc.identifier.issn | 1460-2075 | es |
dc.identifier.uri | https://hdl.handle.net/11441/125556 | |
dc.description.abstract | Tudor staphylococcal nuclease (TSN; also known as Tudor-SN, p100, or SND1) is a multifunctional, evolutionarily conserved regulator of gene expression, exhibiting cytoprotective activity in animals and plants and oncogenic activity in mammals. During stress, TSN stably associates with stress granules (SGs), in a poorly understood process. Here, we show that in the model plant Arabidopsis thaliana, TSN is an intrinsically disordered protein (IDP) acting as a scaffold for a large pool of other IDPs, enriched for conserved stress granule components as well as novel or plant-specific SG-localized proteins. While approximately 30% of TSN interactors are recruited to stress granules de novo upon stress perception, 70% form a protein–protein interaction network present before the onset of stress. Finally, we demonstrate that TSN and stress granule formation promote heat-induced activation of the evolutionarily conserved energy-sensing SNF1-related protein kinase 1 (SnRK1), the plant orthologue of mammalian AMP-activated protein kinase (AMPK). Our results establish TSN as a docking platform for stress granule proteins, with an important role in stress signalling. | es |
dc.description.sponsorship | European Commission 702473 | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad IJCI-2016-30763, PGC2018-099048-B-100 | es |
dc.description.sponsorship | Universidad de Sevilla VIPPIT-2020-IV.4, VIPPIT-2020-I.5 | es |
dc.description.sponsorship | Knut and Alice Wallenberg Foundation 2018.0026 | es |
dc.description.sponsorship | Swedish Research Council VR 2019-04250_VR | es |
dc.description.sponsorship | Swedish Foundation for Strategic Research RBP14-0037 | es |
dc.format | application/pdf | es |
dc.format.extent | 21 p. | es |
dc.language.iso | eng | es |
dc.publisher | Wiley-Blackwell | es |
dc.relation.ispartof | EMBO Journal, 40 (17), e105043. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Heat stress | es |
dc.subject | Intrinsically disordered regions | es |
dc.subject | SnRK1/SNF1/AMPK | es |
dc.subject | Stress granules | es |
dc.subject | Tudor staphylococcal nuclease | es |
dc.title | Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | 702473 | es |
dc.relation.projectID | IJCI-2016-30763 | es |
dc.relation.projectID | PGC2018-099048-B-100 | es |
dc.relation.projectID | VIPPIT-2020-IV.4 | es |
dc.relation.projectID | VIPPIT-2020-I.5 | es |
dc.relation.projectID | 2018.0026 | es |
dc.relation.projectID | 2019-04250_VR | es |
dc.relation.projectID | RBP14-0037 | es |
dc.relation.publisherversion | https://doi.org/10.15252/embj.2020105043 | es |
dc.identifier.doi | 10.15252/embj.2020105043 | es |
dc.journaltitle | EMBO Journal | es |
dc.publication.volumen | 40 | es |
dc.publication.issue | 17 | es |
dc.publication.initialPage | e105043 | es |