Article
Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis
Author/s | Gutiérrez Beltrán, Emilio
Elander, Pernilla H. Dalman, Kerstin Dayhoff, Guy W. II Moschou, Panagiotis N. Uversky, Vladimir N. Crespo, José L. Bozhkov, Peter V. |
Department | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Publication Date | 2021 |
Deposit Date | 2021-09-07 |
Published in |
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Abstract | Tudor staphylococcal nuclease (TSN; also known as Tudor-SN, p100, or SND1) is a multifunctional, evolutionarily conserved regulator of gene expression, exhibiting cytoprotective activity in animals and plants and oncogenic ... Tudor staphylococcal nuclease (TSN; also known as Tudor-SN, p100, or SND1) is a multifunctional, evolutionarily conserved regulator of gene expression, exhibiting cytoprotective activity in animals and plants and oncogenic activity in mammals. During stress, TSN stably associates with stress granules (SGs), in a poorly understood process. Here, we show that in the model plant Arabidopsis thaliana, TSN is an intrinsically disordered protein (IDP) acting as a scaffold for a large pool of other IDPs, enriched for conserved stress granule components as well as novel or plant-specific SG-localized proteins. While approximately 30% of TSN interactors are recruited to stress granules de novo upon stress perception, 70% form a protein–protein interaction network present before the onset of stress. Finally, we demonstrate that TSN and stress granule formation promote heat-induced activation of the evolutionarily conserved energy-sensing SNF1-related protein kinase 1 (SnRK1), the plant orthologue of mammalian AMP-activated protein kinase (AMPK). Our results establish TSN as a docking platform for stress granule proteins, with an important role in stress signalling. |
Project ID. | 702473
IJCI-2016-30763 PGC2018-099048-B-100 VIPPIT-2020-IV.4 VIPPIT-2020-I.5 2018.0026 2019-04250_VR RBP14-0037 |
Citation | Gutiérrez Beltrán, E., Elander, P.H., Dalman, K., Dayhoff, G.W.I., Moschou, P.N., Uversky, V.N.,...,Bozhkov, P.V. (2021). Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis. EMBO Journal, 40 (17), e105043. |
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