dc.creator | Bayona Feliu, Aleix | es |
dc.creator | Barroso Ceballos, Sonia Inés | es |
dc.creator | Muñoz Sánchez, Sergio | es |
dc.creator | Aguilera López, Andrés | es |
dc.date.accessioned | 2021-09-02T14:13:52Z | |
dc.date.available | 2021-09-02T14:13:52Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Bayona Feliu, A., Barroso Ceballos, S.I., Muñoz Sánchez, S. y Aguilera López, A. (2021). The SWI/SNF chromatin remodeling complex helps resolve R-loop-mediated transcription–replication conflicts. Nature Genetics, 53 (7), 1050-1063. | |
dc.identifier.issn | 1061-4036 | es |
dc.identifier.issn | 1546-1718 | es |
dc.identifier.uri | https://hdl.handle.net/11441/125298 | |
dc.description.abstract | ATP-dependent chromatin remodelers are commonly mutated in human cancer. Mammalian SWI/SNF complexes comprise three conserved multisubunit chromatin remodelers (cBAF, ncBAF and PBAF) that share the BRG1 (also known as SMARCA4) subunit responsible for the main ATPase activity. BRG1 is the most frequently mutated Snf2-like ATPase in cancer. In the present study, we have investigated the role of SWI/SNF in genome instability, a hallmark of cancer cells, given its role in transcription, DNA replication and DNA-damage repair. We show that depletion of BRG1 increases R-loops and R-loop-dependent DNA breaks, as well as transcription–replication (T-R) conflicts. BRG1 colocalizes with R-loops and replication fork blocks, as determined by FANCD2 foci, with BRG1 depletion being epistatic to FANCD2 silencing. Our study, extended to other components of SWI/SNF, uncovers a key role of the SWI/SNF complex, in particular cBAF, in helping resolve R-loop-mediated T-R conflicts, thus, unveiling a new mechanism by which chromatin remodeling protects genome integrity. | es |
dc.description.sponsorship | European Research Council ERC2014 AdG669898 TARLOOP | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad BFU2016-75058-P | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación PDI2019-104270GB-I00/BMC | es |
dc.format | application/pdf | es |
dc.format.extent | 58 p. | es |
dc.language.iso | eng | es |
dc.publisher | Springer Nature | es |
dc.relation.ispartof | Nature Genetics, 53 (7), 1050-1063. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | SWI/SNF | es |
dc.subject | Chromatin remodelers | es |
dc.subject | R-loops | es |
dc.subject | Transcription-replication conflicts | es |
dc.subject | BRG1 | es |
dc.subject | Tumor suppressors | es |
dc.subject | cBAF | es |
dc.subject | Fanconi Anemia | es |
dc.title | The SWI/SNF chromatin remodeling complex helps resolve R-loop-mediated transcription–replication conflicts | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/submittedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | ERC2014 AdG669898 TARLOOP | es |
dc.relation.projectID | BFU2016-75058-P | es |
dc.relation.projectID | PDI2019-104270GB-I00/BMC | es |
dc.relation.publisherversion | https://doi.org/10.1038/s41588-021-00867-2 | es |
dc.identifier.doi | 10.1038/s41588-021-00867-2 | es |
dc.journaltitle | Nature Genetics | es |
dc.publication.volumen | 53 | es |
dc.publication.issue | 7 | es |
dc.publication.initialPage | 1050 | es |
dc.publication.endPage | 1063 | es |
dc.description.awardwinning | Premio Anual Publicación Científica Destacada de la US. Facultad de Biología | |