dc.creator | Arellano Orden, Elena | es |
dc.creator | Calero Acuña, Carmen | es |
dc.creator | Moreno Mata, Nicolás | es |
dc.creator | Gómez Izquierdo, Lourdes | es |
dc.creator | Sánchez López, Verónica | es |
dc.creator | López Ramírez, Cecilia | es |
dc.creator | López-Villalobos, José Luis | es |
dc.creator | López-Campos Bodineau, José Luis | es |
dc.date.accessioned | 2021-07-06T14:38:17Z | |
dc.date.available | 2021-07-06T14:38:17Z | |
dc.date.issued | 2016-04-08 | |
dc.identifier.citation | Arellano Orden, E., Calero Acuña, C., Moreno Mata, N., Gómez Izquierdo, L., Sánchez López, V., López Ramírez, C.,...,López-Campos Bodineau, J.L. (2016). Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells. PLoS ONE, 11 (4), art. n.0152737. | |
dc.identifier.issn | 1932-6203 (electrónico) | es |
dc.identifier.uri | https://hdl.handle.net/11441/115256 | |
dc.description.abstract | Background
Conflicting data exist on the role of pulmonary dendritic cells (DCs) and their maturation in patients with chronic obstructive pulmonary disease (COPD). Herein, we investigated whether disease severity and smoking status could affect the distribution and maturation of DCs in lung tissues of patients undergoing elective pneumectomy or lobectomy for suspected primary lung cancer.
Materials and Methods
A total of 75 consecutive patients were included. Spirometry testing was used to identify COPD. Lung parenchyma sections anatomically distant from the primary lesion were examined. We used flow cytometry to identify different DCs subtypes—including BDCA1-positive myeloid DCs (mDCs), BDCA3-positive mDCs, and plasmacytoid DCs (pDCs)—and determine their maturation markers (CD40, CD80, CD83, and CD86) in all participants. We also identified follicular DCs (fDCs), Langerhans DCs (LDCs), and pDCs in 42 patients by immunohistochemistry.
Results
COPD was diagnosed in 43 patients (16 current smokers and 27 former smokers), whereas the remaining 32 subjects were classified as non-COPD (11 current smokers, 13 former smokers, and 8 never smokers). The number and maturation of DCs did not differ significantly between COPD and non-COPD patients. However, the results of flow cytometry indicated that maturation markers CD40 and CD83 of BDCA1-positive mDCs were significantly decreased in smokers than in non-smokers (P = 0.023 and 0.013, respectively). Immunohistochemistry also revealed a lower number of LDCs in COPD patients than in non-COPD subjects.
Conclusions
Cigarette smoke, rather than airflow limitation, is the main determinant of impaired DCs maturation in the lung. | es |
dc.description.sponsorship | Instituto de Salud Carlos III | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad | es |
dc.description.sponsorship | Fondo de Investigación Sanitaria (project PS09/00826) | es |
dc.format | application/pdf | es |
dc.format.extent | 15 p. | es |
dc.language.iso | eng | es |
dc.publisher | Public Library of Science | es |
dc.relation.ispartof | PLoS ONE, 11 (4), art. n.0152737. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Cigarette | es |
dc.subject | Smoke | es |
dc.subject | Lung | es |
dc.subject | Myeloid Dendritic Cells | es |
dc.title | Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Cirugía | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.relation.projectID | project PS09/00826 | es |
dc.relation.publisherversion | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0152737 | es |
dc.identifier.doi | 10.1371/journal.pone.0152737 | es |
dc.journaltitle | PLoS ONE | es |
dc.publication.volumen | 11 | es |
dc.publication.issue | 4 | es |
dc.publication.initialPage | art. n.0152737 | es |