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dc.creatorArellano Orden, Elenaes
dc.creatorCalero Acuña, Carmenes
dc.creatorMoreno Mata, Nicoláses
dc.creatorGómez Izquierdo, Lourdeses
dc.creatorSánchez López, Verónicaes
dc.creatorLópez Ramírez, Ceciliaes
dc.creatorLópez-Villalobos, José Luises
dc.creatorLópez-Campos Bodineau, José Luises
dc.date.accessioned2021-07-06T14:38:17Z
dc.date.available2021-07-06T14:38:17Z
dc.date.issued2016-04-08
dc.identifier.citationArellano Orden, E., Calero Acuña, C., Moreno Mata, N., Gómez Izquierdo, L., Sánchez López, V., López Ramírez, C.,...,López-Campos Bodineau, J.L. (2016). Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells. PLoS ONE, 11 (4), art. n.0152737.
dc.identifier.issn1932-6203 (electrónico)es
dc.identifier.urihttps://hdl.handle.net/11441/115256
dc.description.abstractBackground Conflicting data exist on the role of pulmonary dendritic cells (DCs) and their maturation in patients with chronic obstructive pulmonary disease (COPD). Herein, we investigated whether disease severity and smoking status could affect the distribution and maturation of DCs in lung tissues of patients undergoing elective pneumectomy or lobectomy for suspected primary lung cancer. Materials and Methods A total of 75 consecutive patients were included. Spirometry testing was used to identify COPD. Lung parenchyma sections anatomically distant from the primary lesion were examined. We used flow cytometry to identify different DCs subtypes—including BDCA1-positive myeloid DCs (mDCs), BDCA3-positive mDCs, and plasmacytoid DCs (pDCs)—and determine their maturation markers (CD40, CD80, CD83, and CD86) in all participants. We also identified follicular DCs (fDCs), Langerhans DCs (LDCs), and pDCs in 42 patients by immunohistochemistry. Results COPD was diagnosed in 43 patients (16 current smokers and 27 former smokers), whereas the remaining 32 subjects were classified as non-COPD (11 current smokers, 13 former smokers, and 8 never smokers). The number and maturation of DCs did not differ significantly between COPD and non-COPD patients. However, the results of flow cytometry indicated that maturation markers CD40 and CD83 of BDCA1-positive mDCs were significantly decreased in smokers than in non-smokers (P = 0.023 and 0.013, respectively). Immunohistochemistry also revealed a lower number of LDCs in COPD patients than in non-COPD subjects. Conclusions Cigarette smoke, rather than airflow limitation, is the main determinant of impaired DCs maturation in the lung.es
dc.description.sponsorshipInstituto de Salud Carlos IIIes
dc.description.sponsorshipMinisterio de Economía y Competitividades
dc.description.sponsorshipFondo de Investigación Sanitaria (project PS09/00826)es
dc.formatapplication/pdfes
dc.format.extent15 p.es
dc.language.isoenges
dc.publisherPublic Library of Sciencees
dc.relation.ispartofPLoS ONE, 11 (4), art. n.0152737.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCigarettees
dc.subjectSmokees
dc.subjectLunges
dc.subjectMyeloid Dendritic Cellses
dc.titleCigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cellses
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Cirugíaes
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Medicinaes
dc.relation.projectIDproject PS09/00826es
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0152737es
dc.identifier.doi10.1371/journal.pone.0152737es
dc.journaltitlePLoS ONEes
dc.publication.volumen11es
dc.publication.issue4es
dc.publication.initialPageart. n.0152737es

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