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dc.creatorGarcía Quintanilla, Meritxell de Jesúses
dc.creatorPulido, Marina R.es
dc.creatorPachón Díaz, Jerónimoes
dc.creatorMcConnell, MJes
dc.date.accessioned2021-05-27T10:16:52Z
dc.date.available2021-05-27T10:16:52Z
dc.date.issued2014
dc.identifier.citationGarcía Quintanilla, M.d.J., Pulido Toledano, M.R., Pachón Díaz, J. y McConnell, M. (2014). Immunization with Lipopolysaccharide-Deficient Whole Cells Provides Protective Immunity in an Experimental Mouse Model of Acinetobacter baumannii Infection. PLoS One, 9 (12), 1-14.
dc.identifier.issn1932-6203es
dc.identifier.urihttps://hdl.handle.net/11441/110885
dc.descriptionEditor: Gunnar F. Kaufmannes
dc.description.abstractThe increasing clinical importance of infections caused by multidrug resistant Acinetobacter baumannii warrants the development of novel approaches for prevention and treatment. In this context, vaccination of certain patient populations may contribute to reducing the morbidity and mortality caused by this pathogen. Vaccines against Gram-negative bacteria based on inactivated bacterial cells are highly immunogenic and have been shown to produce protective immunity against a number of bacterial species. However, the high endotoxin levels present in these vaccines due to the presence of lipopolysaccharide complicates their use in human vaccination. In the present study, we used a laboratory-derived strain of A. baumannii that completely lacks lipopolysaccharide due to a mutation in the lpxD gene (IB010), one of the genes involved in the first steps of lipopolysaccharide biosynthesis, for vaccination. We demonstrate that IB010 has greatly reduced endotoxin content (,1.0 endotoxin unit/106 cells) compared to wild type cells. Immunization with formalin inactivated IB010 produced a robust antibody response consisting of both IgG1 and IgG2c subtypes. Mice immunized with IB010 had significantly lower post-infection tissue bacterial loads and significantly lower serum levels of the pro-inflammatory cytokines IL-1b, TNF-a and IL-6 compared to control mice in a mouse model of disseminated A. baumannii infection. Importantly, immunized mice were protected from infection with the ATCC 19606 strain and an A. baumannii clinical isolate. These data suggest that immunization with inactivated A. baumannii whole cells deficient in lipopolysaccharide could serve as the basis for a vaccine for the prevention of infection caused by A. baumannii.es
dc.description.sponsorshipREIPI REIPI RD06/0008/0000es
dc.description.sponsorshipConsejería de Salud de la Junta de Andalucía (PI-0046-2011)es
dc.description.sponsorshipMinisterio de Economía y Competitividad, Subprograma Miguel Servet (CP11/00314)es
dc.formatapplication/pdfes
dc.format.extent14es
dc.language.isoenges
dc.relation.ispartofPLoS One, 9 (12), 1-14.
dc.rightsAtribución-Reconocimiento 4.0 internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAcinetobacter baumanniies
dc.subjectVaccineses
dc.subjectMorbidityes
dc.subjectMortalityes
dc.subjectGram-negative bacteriaes
dc.titleImmunization with Lipopolysaccharide-Deficient Whole Cells Provides Protective Immunity in an Experimental Mouse Model of Acinetobacter baumannii Infectiones
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiologíaes
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Medicinaes
dc.identifier.doi10.1371/journal.pone. 0114410es
dc.contributor.groupUniversidad de Sevilla. CTS-203: Estudio de las Enfermedades Infecciosases
dc.journaltitlePLoS Onees
dc.publication.volumen9es
dc.publication.issue12es
dc.publication.initialPage1es
dc.publication.endPage14es

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