miR-30b-5p Downregulation as a predictive biomarker of coronary in-stent restenosis
Mayoral González, Isabel
Morón, Francisco Jesús
Domínguez Rodríguez, Alejandro
Smani Hajami, Tarik
|Department||Universidad de Sevilla. Departamento de Cirugía
Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica
|Published in||Biomedicines, 9 (4)|
|Abstract||In-stent restenosis (ISR) is one of the main limitations of percutaneous coronary intervention (PCI) therapy with drug-eluting stents (DES) implantation. The aim of this study was to determine if circulating microRNAs ...
In-stent restenosis (ISR) is one of the main limitations of percutaneous coronary intervention (PCI) therapy with drug-eluting stents (DES) implantation. The aim of this study was to determine if circulating microRNAs (miRNAs) have diagnostic capability for determining ISR in a cohort of matched patients. Blood samples were collected from 55 patients who underwent previously PCI and were readmitted for a new coronary angiography. Patients were divided into subgroups comprising patients who presented ISR or not (non-ISR). A microarray analysis determined that up to 49 miRNAs were differentially expressed between ISR and non-ISR patients. Of these, 10 miRNAs are related to vascular smooth muscle and endothelial cells proliferation, migration, and differentiation, well-known hallmarks of vascular remodeling. Additionally, we identified that the expression of miR-30b-5p is significantly lower in serum samples of ISR patients, as compared to non-ISR. A further analysis demonstrated that miR-30b-5p provides better values of the receiver operator characteristic curve than other miRNAs and biochemical parameters. Finally, the in-silico analysis suggests that miR-30b-5p is predicted to target 62 genes involved in different signaling pathways involved in vascular remodeling. In conclusion, we determined for the first time that circulating mi-R30b-5p can reliably prognose restenosis in patient with implanted DES, which could be potentially helpful in the establishment of an early diagnosis and therapy of ISR.
|Cite||Gutierrez-Carretero, E., Mayoral González, I., Morón, F.J., Fernandez-Quero, M., Domínguez Rodríguez, A., Ordoñez, A. y Smani Hajami, T. (2021). miR-30b-5p Downregulation as a predictive biomarker of coronary in-stent restenosis4. Biomedicines, 9 (4)|