dc.creator | Olaya-Abril, Alonso | es |
dc.creator | Jiménez-Munguía, Irene | es |
dc.creator | Gómez-Gascón, Lidia | es |
dc.creator | Obando Santaella, Ignacio | es |
dc.creator | Rodríguez Ortega, Manuel J. | es |
dc.date.accessioned | 2021-04-30T15:57:15Z | |
dc.date.available | 2021-04-30T15:57:15Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Olaya-Abril, A., Jiménez-Munguía, I., Gómez-Gascón, L., Obando Santaella, I. y Rodríguez Ortega, M.J. (2015). A Pneumococcal Protein Array as a Platform to Discover Serodiagnostic Antigens Against Infection. Molecular And Cellular Proteomics, 14 (10), 2591-2608. | |
dc.identifier.issn | 1535-9476 | es |
dc.identifier.uri | https://hdl.handle.net/11441/108274 | |
dc.description.abstract | Pneumonia is one of the most common and severe diseases associated with Streptococcus pneumoniae infections in children and adults. Etiological diagnosis of pneumococcal pneumonia in children is generally challenging
because of limitations of diagnostic tests and interference
with nasopharyngeal colonizing strains. Serological assays have recently gained interest to overcome some
problems found with current diagnostic tests in pediatric
pneumococcal pneumonia. To provide insight into this
field, we have developed a protein array to screen the
antibody response to many antigens simultaneously. Proteins were selected by experimental identification from a
collection of 24 highly prevalent pediatric clinical isolates
in Spain, using a proteomics approach consisting of
“shaving” the cell surface with proteases and further LC/
MS/MS analysis. Ninety-five proteins were recombinantly
produced and printed on an array. We probed it with a
collection of sera from children with pneumococcal
pneumonia. From the set of the most seroprevalent
antigens, we obtained a clear discriminant response
for a group of three proteins (PblB, PulA, and PrtA) in
children under 4 years old. We validated the results by
ELISA and an immunostrip assay showed the translation
to easy-to-use, affordable tests. Thus, the protein array
here developed presents a tool for broad use in
serodiagnostics. | es |
dc.format | application/pdf | es |
dc.format.extent | 18 p. | es |
dc.language.iso | eng | es |
dc.relation.ispartof | Molecular And Cellular Proteomics, 14 (10), 2591-2608. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Streptococcus pneumoniae | es |
dc.subject | Pneumonia | es |
dc.subject | PblB | es |
dc.subject | PulA | es |
dc.subject | PrtA | es |
dc.title | A Pneumococcal Protein Array as a Platform to Discover Serodiagnostic Antigens Against Infection | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Farmacología, Pediatría y Radiología | es |
dc.identifier.doi | 10.1074/mcp.M115.049544 | es |
dc.journaltitle | Molecular And Cellular Proteomics | es |
dc.publication.volumen | 14 | es |
dc.publication.issue | 10 | es |
dc.publication.initialPage | 2591 | es |
dc.publication.endPage | 2608 | es |