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dc.creatorHerrera Espejo, Sorayaes
dc.creatorCebrero Cangueiro, Taniaes
dc.creatorLabrador Herrera, Gemaes
dc.creatorPachón Díaz, Jerónimoes
dc.creatorPachón Ibáñez, María Eugeniaes
dc.creatorÁlvarez Marín, Rocíoes
dc.date.accessioned2021-02-11T11:53:22Z
dc.date.available2021-02-11T11:53:22Z
dc.date.issued2020-12-09
dc.identifier.citationHerrera Espejo, S., Cebrero Cangueiro, T., Labrador Herrera, G., Pachón, J., Pachón Ibáñez, M.E. y Álvarez Marín, R. (2020). In vitro activity of pentamidine alone and in combination with antibiotics against multidrug-resistant clinical Pseudomonas aeruginosa strains. Antibiotics, 9 (12), 1-12.
dc.identifier.issn2079-6382es
dc.identifier.urihttps://hdl.handle.net/11441/104859
dc.description.abstractMultidrug-resistant (MDR) Pseudomonas aeruginosa is a public health problem causing both community and hospital-acquired infections, and thus the development of new therapies for these infections is critical. The objective of this study was to analyze in vitro the activity of pentamidine as adjuvant in combinations to antibiotics against seven clinical P. aeruginosa strains. The Minimum Inhibitory Concentration (MIC) was determined following standard protocols, and the results were interpreted according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints; however, the gentamicin activity was interpreted according to the Clinical and Laboratory Standards Institute (CLSI) recommendations. The bactericidal in vitro activity was studied at 1×MIC concentrations by time–kill curves, and also performed in three selected strains at 1/2×MIC of pentamidine. All studies were performed in triplicate. The pentamidine MIC range was 400–1600 µg/mL. Four of the strains were MDR, and the other three were resistant to two antibiotic families. The combinations of pentamidine at 1×MIC showed synergistic activity against all the tested strains, except for pentamidine plus colistin. Pentamidine plus imipenem and meropenem were the combinations that showed synergistic activity against the most strains. At 1/2×MIC, pentamidine plus antibiotics were synergistic with all three analyzed strains. In summary, pentamidine in combination with antibiotics showed in vitro synergy against multidrug-resistant P. aeruginosa clinical strains, which suggests its possible use as adjuvant to antibiotics for the therapy of infections from MDR P. aeruginosa.es
dc.description.sponsorshipInstituto de Salud Carlos III Proyectos de Investigación en Salud PI18-01842es
dc.description.sponsorshipSubdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economía, Industria y Competitividad, Red Española de Investigación en Enfermedades Infecciosas REIPI RD16 / 0016/0009es
dc.description.sponsorshipFondo Regional de Desarrollo Europeo Una forma de lograr Europa, Operativa programa Crecimiento inteligente 2014-2020. T.Ces
dc.description.sponsorshipUniversidad de Sevilla. Servicio Andaluz de Salud, Junta de Andalucía C1-0038-2019es
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades, Instituto de Salud Carlos III, cofinanciado por la Unión Fondo Regional de Desarrollo RD16 / 0016/0009es
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades, Instituto de Salud Carlos III, cofinanciado por European Development Regional Fund (A Way to Achieve Europe) y por la Red Española de Investigación en Enfermedades Infecciosas JR17 / 00025es
dc.formatapplication/pdfes
dc.format.extent12es
dc.language.isoenges
dc.publisherMDPIes
dc.relation.ispartofAntibiotics, 9 (12), 1-12.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectPseudomonas aeruginosaes
dc.subjectMultidrug-resistancees
dc.subjectAntibioticses
dc.subjectPentamidinees
dc.subjectIn vitro activityes
dc.titleIn vitro activity of pentamidine alone and in combination with antibiotics against multidrug-resistant clinical Pseudomonas aeruginosa strainses
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Medicinaes
dc.contributor.affiliationInstituto de Biomedicina de Sevilla (IBIS)es
dc.relation.projectIDPI18-01842es
dc.relation.projectIDRD16 / 0016/0009es
dc.relation.projectID2014-2020. T.Ces
dc.relation.projectIDC1-0038-2019es
dc.relation.projectIDRD16 / 0016/0009es
dc.relation.projectIDJR17 / 00025es
dc.relation.publisherversionhttps://doi.org/10.3390/antibiotics9120885es
dc.identifier.doi10.3390/antibiotics9120885es
dc.journaltitleAntibioticses
dc.publication.volumen9es
dc.publication.issue12es
dc.publication.initialPage1es
dc.publication.endPage12es

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