Sinorhizobium fredii HH103 invades lotus burttii by crack entry in a nod factor- and surface polysaccharide-dependent manner
|Author/s||Acosta Jurado, Sebastián
Rodríguez-Navarro, Dulce Nombre
Perea, Juan Fernández
Gil Serrano, Antonio Miguel
Rodríguez Carvajal, Miguel Ángel
Andersen, Stig Uggerhøj
Sandal, Niels Nørgaard
Vinardell González, José María
Ruiz Sainz, José Enrique
|Department||Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Química orgánica
|Abstract||Sinorhizobium fredii HH103-Rifr, a broad host range rhizobial strain, induces nitrogen-fixing nodules in Lotus burttii but ineffective nodules in L. japonicus. Confocal microscopy studies showed that Mesorhizobium loti ...
Sinorhizobium fredii HH103-Rifr, a broad host range rhizobial strain, induces nitrogen-fixing nodules in Lotus burttii but ineffective nodules in L. japonicus. Confocal microscopy studies showed that Mesorhizobium loti MAFF303099 and S. fredii HH103-Rifr invade L. burttii roots through infection threads or epidermal cracks, respectively. Infection threads in root hairs were not observed in L. burttii plants inoculated with S. fredii HH103-Rifr. A S. fredii HH103-Rifr nodA mutant failed to nodulate L. burttii, demonstrating that Nod factors are strictly necessary for this crack-entry mode, and a noeL mutant was also severely impaired in L. burttii nodulation, indicating that the presence of fucosyl residues in the Nod factor is symbiotically relevant. However, significant symbiotic impacts due to the absence of methylation or to acetylation of the fucosyl residue were not detected. In contrast S. fredii HH103-Rifr mutants showing lipopolysaccharide alterations had reduced symbiotic capacity, while mutants affected in production of either exopolysaccharides, capsular polysaccharides, or both were not impaired in nodulation. Mutants unable to produce cyclic glucans and purine or pyrimidine auxotrophic mutants formed ineffective nodules with L. burttii. Flagellin-dependent bacterial mobility was not required for crack infection, since HH103-Rifr fla mutants nodulated L. burttii. None of the S. fredii HH103-Rif r surface-polysaccharide mutants gained effective nodulation with L. japonicus.
|Citation||Acosta Jurado, S., Rodríguez-Navarro, D.N., Kawaharada, Y., Perea, J.F., Gil Serrano, A.M., Jin, H.,...,Ruiz Sainz, J.E. (2016). Sinorhizobium fredii HH103 invades lotus burttii by crack entry in a nod factor- and surface polysaccharide-dependent manner. Molecular Plant-Microbe Interactions, 29 (12), 925-937.|