dc.creator | Harding, Christian M. | es |
dc.creator | Pulido, Marina R. | es |
dc.creator | Venanzio, Gisela Di | es |
dc.creator | Kinsella, Rachel L. | es |
dc.creator | Webb, Andrew I. | es |
dc.creator | Scott, Nichollas E. | es |
dc.creator | Pachón Díaz, Jerónimo | es |
dc.creator | Feldman, Mario F. | es |
dc.date.accessioned | 2020-10-23T18:52:12Z | |
dc.date.available | 2020-10-23T18:52:12Z | |
dc.date.issued | 2017-04-03 | |
dc.identifier.citation | Harding, C.M., Pulido, M.R., Venanzio, G.D., Kinsella, R.L., Webb, A.I., Scott, N.E.,...,Feldman, M.F. (2017). Pathogenic Acinetobacter species have a functional type I secretion system and contact-dependent inhibition systems. Journal of Biological Chemistry, 292 (22), 9075-9087. https://doi.org/10.1074/jbc.M117.781575. | |
dc.identifier.issn | 0021-9258 | es |
dc.identifier.issn | 1083-351X | es |
dc.identifier.uri | https://hdl.handle.net/11441/102213 | |
dc.description.abstract | Pathogenic Acinetobacter species, including Acinetobacter
baumannii and Acinetobacter nosocomialis, are opportunistic
human pathogens of increasing relevance worldwide. Although
their mechanisms of drug resistance are well studied, the virulence factors that governAcinetobacter pathogenesis are incompletely characterized. Here we define the complete secretome of
A. nosocomialis strain M2 in minimal medium and demonstrate
that pathogenicAcinetobacter species produce both a functional
type I secretion system (T1SS) and a contact-dependent inhibition (CDI) system. Using bioinformatics, quantitative proteomics, and mutational analyses, we show that Acinetobacter uses
its T1SS for exporting two putative T1SS effectors, an Repeatsin-Toxin (RTX)-serralysin-like toxin, and the biofilm-associated protein (Bap). Moreover, we found that mutation of any
component of the T1SS system abrogated type VI secretion
activity under nutrient-limited conditions, indicating a previously unrecognized cross-talk between these two systems. We
also demonstrate that the Acinetobacter T1SS is required for
biofilm formation. Last, we show that both A. nosocomialis and
A. baumannii produce functioning CDI systems that mediate
growth inhibition of sister cells lacking the cognate immunity
protein. The Acinetobacter CDI systems are widely distributed
across pathogenicAcinetobacter species, with manyA. baumannii isolates harboring two distinct CDI systems. Collectively,
these data demonstrate the power of differential, quantitative
proteomics approaches to study secreted proteins, define the
role of previously uncharacterized protein export systems, and
observe cross-talk between secretion systems in the pathobiology of medically relevant Acinetobacter species | es |
dc.description.sponsorship | Subprograma Sara Borrell from the Instituto de Salud Carlos III | es |
dc.description.sponsorship | Subdirección General de Evaluación y Fomento de la Investigación | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad de España CD14/00014 | es |
dc.format | application/pdf | es |
dc.format.extent | 13 | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Biochemistry and Molecular Biology, Inc. | es |
dc.relation.ispartof | Journal of Biological Chemistry, 292 (22), 9075-9087. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Pathogenic Acinetobacter | es |
dc.subject | Acinetobacter baumannii | es |
dc.subject | Acinetobacter nosocomialis | es |
dc.title | Pathogenic Acinetobacter species have a functional type I secretion system and contact-dependent inhibition systems | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.relation.projectID | CD14/00014 | es |
dc.identifier.doi | 10.1074/jbc.M117.781575 | |
dc.journaltitle | Journal of Biological Chemistry | es |
dc.publication.volumen | 292 | es |
dc.publication.issue | 22 | es |
dc.publication.initialPage | 9075 | es |
dc.publication.endPage | 9087 | es |