dc.creator | Fernandez-Garcia, Laura | es |
dc.creator | Ambroa, A. | es |
dc.creator | Blasco, L. | es |
dc.creator | Bleriot, Inés | es |
dc.creator | López, María | es |
dc.creator | Álvarez-Marín, Rocío | es |
dc.creator | Rodríguez-Baño, Jesús | es |
dc.creator | Pachón Díaz, Jerónimo | es |
dc.creator | Tomás, María | es |
dc.date.accessioned | 2020-10-06T18:27:26Z | |
dc.date.available | 2020-10-06T18:27:26Z | |
dc.date.issued | 2018-12-17 | |
dc.identifier.citation | Fernandez-Garcia, L., Ambroa, A., Blasco, L., Bleriot, I., López, M., Alvarez-Marin, R.,...,Tomás, M. (2018). Relationship Between the Quorum Network (Sensing/Quenching) and Clinical Features of Pneumonia and Bacteraemia Caused by A. baumannii. Frontiers in microbiology, 9 | |
dc.identifier.issn | 1664-302X | es |
dc.identifier.uri | https://hdl.handle.net/11441/101754 | |
dc.description.abstract | Acinetobacter baumannii (Ab) is one of the most important pathogens associated
with nosocomial infections, especially pneumonia. Interest in the Quorum network,
i.e., Quorum Sensing (QS)/Quorum Quenching (QQ), in this pathogen has grown
in recent years. The Quorum network plays an important role in regulating diverse
virulence factors such as surface motility and bacterial competition through the
type VI secretion system (T6SS), which is associated with bacterial invasiveness.
In the present study, we investigated 30 clinical strains of A. baumannii isolated in
the “II Spanish Study of A. baumannii GEIH-REIPI 2000-2010” (Genbank Umbrella
Bioproject PRJNA422585), a multicentre study describing the relationship between the
Quorum network in A. baumannii and the development of pneumonia and associated
bacteraemia. Expression of the aidA gene (encoding the AidA protein, QQ enzyme) was
lower (P < 0.001) in strains of A. baumannii isolated from patients with bacteraemic
pneumonia than in strains isolated from patients with non-bacteraemic pneumonia.
Moreover, aidA expression in the first type of strain was not regulated in the presence
of environmental stress factors such as the 3-oxo-C12-HSL molecule (substrate of AidA
protein, QQ activation) or H2O2 (inhibitor of AidA protein, QS activation). However, in the
A. baumannii strains isolated from patients with non-bacteraemic pneumonia, aidA gene
expression was regulated by stressors such as 3-oxo-C12-HSL and H2O2. In an in vivo
Galleria mellonella model of A. baumannii infection, the A. baumannii ATCC 17978 strain
was associated with higher mortality (100% at 24 h) than the mutant, abaI-deficient, strain (carrying a synthetase enzyme of Acyl homoserine lactone molecules) (70% at 24 h).
These data suggest that the QS (abaR and abaI genes)/QQ (aidA gene) network affects
the development of secondary bacteraemia in pneumonia patients and also the virulence
of A. baumannii. | es |
dc.description.sponsorship | National Plan for Scientific Research | es |
dc.description.sponsorship | Technological Development and Innovation PI16/01163 | es |
dc.description.sponsorship | ISCIII-Deputy General Directorate for Evaluation and Promotion of Research-European Regional Development Fund A way of Making Europe | es |
dc.description.sponsorship | Instituto de Salud Carlos III | es |
dc.description.sponsorship | Miguel Servet Research Programme SERGAS and ISCIII | es |
dc.description.sponsorship | Xunta de Galicia (GAIN, Axencia de Innovación) | es |
dc.format | application/pdf | es |
dc.format.extent | 11 | es |
dc.language.iso | eng | es |
dc.publisher | Frontiers Research Foundation | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Quorum | es |
dc.subject | Pneumonia | es |
dc.subject | Sensing | es |
dc.subject | Quenching | es |
dc.subject | Bacteraemia | es |
dc.subject | Acinetobacter | es |
dc.title | Relationship Between the Quorum Network (Sensing/Quenching) and Clinical Features of Pneumonia and Bacteraemia Caused by A. baumannii | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.relation.projectID | PI16/01163 | es |
dc.relation.projectID | RD16/0016/0001 | es |
dc.relation.projectID | RD16/0016/0006 | es |
dc.relation.projectID | RD16/0016/0008 | es |
dc.relation.projectID | RD16/0016/0009 | es |
dc.relation.projectID | RD16/0016/0010 | es |
dc.identifier.doi | 10.3389/fmicb.2018.03105 | es |
dc.journaltitle | Frontiers in microbiology | es |
dc.publication.volumen | 9 | es |