Artículo
Aquaporin-4 removal from the plasma membrane of human muller cells by AQP4-IgG from patients with neuromyelitis optica induces changes in cell volume homeostasis: the first step of retinal injury?
Autor/es | Netti, Vanina
Fernández, Juan Melamud, Luciana García Miranda, Pablo Di Giusto, Gisela Ford, Paula Echevarría Irusta, Miriam Capurro, Claudia |
Departamento | Universidad de Sevilla. Departamento de Fisiología |
Fecha de publicación | 2021-07 |
Fecha de depósito | 2023-12-29 |
Publicado en |
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Resumen | Aquaporin-4 (AQP4) is the target of the specifc immunoglobulin G autoantibody (AQP4-IgG) produced in patients with
neuromyelitis optica spectrum disorders (NMOSD). Previous studies demonstrated that AQP4-IgG binding to ... Aquaporin-4 (AQP4) is the target of the specifc immunoglobulin G autoantibody (AQP4-IgG) produced in patients with neuromyelitis optica spectrum disorders (NMOSD). Previous studies demonstrated that AQP4-IgG binding to astrocytic AQP4 leads to cell-destructive lesions. However, the early physiopathological events in Müller cells in the retina are poorly understood. Here, we investigated the consequences of AQP4-IgG binding to AQP4 of Müller cells, previous to the infammatory response, on two of AQP4’s key functions, cell volume regulation response (RVD) and cell proliferation, a process closely associated with changes in cell volume. Experiments were performed in a human retinal Müller cell line (MIO-M1) exposed to complement-inactivated sera from healthy volunteers or AQP4-IgG positive NMOSD patients. We evaluated AQP4 expression (immunofuorescence and western blot), water permeability coefcient, RVD, intracellular calcium levels and membrane potential changes during hypotonic shock (fuorescence videomicroscopy) and cell proliferation (cell count and BrdU incorporation). Our results showed that AQP4-IgG binding to AQP4 induces its partial internalization, leading to the decrease of the plasma membrane water permeability, a reduction of swelling-induced increase of intracellular calcium levels and the impairment of RVD in Müller cells. The loss of AQP4 from the plasma membrane induced by AQP4-IgG positive sera delayed Müller cells’ proliferation rate. We propose that Müller cell dysfunction after AQP4 removal from the plasma membrane by AQP4-IgG binding could be a non-infammatory mechanism of retinal injury in vivo, altering cell volume homeostasis and cell proliferation and consequently, contributing to the physiopathology of NMOSD. |
Agencias financiadoras | Universidad de Buenos Aires (UBA). Argentina Agencia Nacional de Promoción Científica y Tecnológica. Argentina Ministerio de Economía y Competitividad (MINECO). España Instituto de Salud Carlos III. España European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) |
Identificador del proyecto | UBA-SECYT, 20020130100682BA, 2018–2021
ANPCyT 2019–01707 FEDER, PI16/00493 |
Cita | Netti, V., Fernández, J., Melamud, L., García Miranda, P., Di Giusto, G., Ford, P.,...,Capurro, C. (2021). Aquaporin-4 removal from the plasma membrane of human muller cells by AQP4-IgG from patients with neuromyelitis optica induces changes in cell volume homeostasis: the first step of retinal injury?. Cellular and Molecular Neurobiology, 58 (10), 5178-5193. https://doi.org/10.1007/s12035-021-02491-x. |
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