Artículo
Investigation of the intestinal trans-epithelial transport and antioxidant activity of two hempseed peptides WVSPLAGRT (H2) and IGFLIIWV (H3)
Autor/es | Bollati, Carlotta
Cruz Chamorro, Iván Aiello, Gilda Li, Jianqiang Bartolomei, Martina Santos Sánchez, Guillermo Lammi, Carmen |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología |
Fecha de publicación | 2022 |
Fecha de depósito | 2022-12-16 |
Publicado en |
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Resumen | A preceding paper has shown that a hempseed peptic hydrolysate displays a cholesterol-lowering activity with a
statin-like mechanism of action in HepG2 cells and a potential hypoglycemic activity by the inhibition of ... A preceding paper has shown that a hempseed peptic hydrolysate displays a cholesterol-lowering activity with a statin-like mechanism of action in HepG2 cells and a potential hypoglycemic activity by the inhibition of dipeptidyl peptidase-IV in Caco-2 cells. In the framework of a research aimed at fostering the multifunctional behavior of hempseed peptides, we present here the identification and evaluation of some antioxidant peptides from the same hydrolysate. After evaluation of its diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, a trans-epithelial transport experiment was performed using differentiated Caco-2 cells that permitted the identification of five transported peptides that were synthesized and evaluated by measuring the oxygen radical absorbance capacity (ORAC), the ferric reducing antioxidant power (FRAP), and the 2,2-azino-bis-(3-ethylbenzo thiazoline-6-sulfonic) acid (ABTS), and diphenyl-2-picrylhydrazyl radical DPPH assays. The most active peptides, i.e. WVSPLAGRT (H2) and IGFLIIWV (H3), were then tested in cell assays. Both peptides were able to reduce the H2O2-induced reactive oxygen species (ROS), lipid peroxidation, and nitric oxide (NO) production levels in HepG2 cells, via the modulation of Nrf-2 and iNOS pathways, respectively. |
Cita | Bollati, C., Cruz Chamorro, I., Aiello, G., Li, J., Bartolomei, M., Santos Sánchez, G. y Lammi, C. (2022). Investigation of the intestinal trans-epithelial transport and antioxidant activity of two hempseed peptides WVSPLAGRT (H2) and IGFLIIWV (H3). FOOD RESEARCH INTERNATIONAL, 152, 110720. https://doi.org/10.1016/j.foodres.2021.110720. |
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