dc.creator | Bollati, Carlotta | es |
dc.creator | Cruz Chamorro, Iván | es |
dc.creator | Aiello, Gilda | es |
dc.creator | Li, Jianqiang | es |
dc.creator | Bartolomei, Martina | es |
dc.creator | Santos Sánchez, Guillermo | es |
dc.creator | Lammi, Carmen | es |
dc.date.accessioned | 2022-12-16T15:52:39Z | |
dc.date.available | 2022-12-16T15:52:39Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Bollati, C., Cruz Chamorro, I., Aiello, G., Li, J., Bartolomei, M., Santos Sánchez, G. y Lammi, C. (2022). Investigation of the intestinal trans-epithelial transport and antioxidant activity of two hempseed peptides WVSPLAGRT (H2) and IGFLIIWV (H3). FOOD RESEARCH INTERNATIONAL, 152, 110720. https://doi.org/10.1016/j.foodres.2021.110720. | |
dc.identifier.issn | 0963-9969 | es |
dc.identifier.issn | 1873-7145 | es |
dc.identifier.uri | https://hdl.handle.net/11441/140584 | |
dc.description.abstract | A preceding paper has shown that a hempseed peptic hydrolysate displays a cholesterol-lowering activity with a
statin-like mechanism of action in HepG2 cells and a potential hypoglycemic activity by the inhibition of
dipeptidyl peptidase-IV in Caco-2 cells. In the framework of a research aimed at fostering the multifunctional
behavior of hempseed peptides, we present here the identification and evaluation of some antioxidant peptides
from the same hydrolysate. After evaluation of its diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity,
a trans-epithelial transport experiment was performed using differentiated Caco-2 cells that permitted the
identification of five transported peptides that were synthesized and evaluated by measuring the oxygen radical
absorbance capacity (ORAC), the ferric reducing antioxidant power (FRAP), and the 2,2-azino-bis-(3-ethylbenzo thiazoline-6-sulfonic) acid (ABTS), and diphenyl-2-picrylhydrazyl radical DPPH assays. The most active peptides,
i.e. WVSPLAGRT (H2) and IGFLIIWV (H3), were then tested in cell assays. Both peptides were able to reduce the
H2O2-induced reactive oxygen species (ROS), lipid peroxidation, and nitric oxide (NO) production levels in
HepG2 cells, via the modulation of Nrf-2 and iNOS pathways, respectively. | es |
dc.format | application/pdf | es |
dc.format.extent | 11 p. | es |
dc.language.iso | eng | es |
dc.publisher | ELSEVIER SCIENCE INC | es |
dc.relation.ispartof | FOOD RESEARCH INTERNATIONAL, 152, 110720. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Antioxidant peptides | es |
dc.subject | Bioactive peptides | es |
dc.subject | Hempseed peptides | es |
dc.subject | Nrf-2 | es |
dc.subject | ROS | es |
dc.title | Investigation of the intestinal trans-epithelial transport and antioxidant activity of two hempseed peptides WVSPLAGRT (H2) and IGFLIIWV (H3) | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología | es |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0963996921006190?via%3Dihub | es |
dc.identifier.doi | 10.1016/j.foodres.2021.110720 | es |
dc.contributor.group | Universidad de Sevilla. CTS160: Neuroendocrinología Molecular. | es |
dc.journaltitle | FOOD RESEARCH INTERNATIONAL | es |
dc.publication.volumen | 152 | es |
dc.publication.initialPage | 110720 | es |