dc.creator | Sobrevia Luarte, Luis | es |
dc.creator | Sáez, Tamara | es |
dc.creator | Vos, Paul de | es |
dc.creator | Kuipers, Jeroen | es |
dc.creator | Marijke, M.Faas | es |
dc.date.accessioned | 2019-04-17T11:33:10Z | |
dc.date.available | 2019-04-17T11:33:10Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Sobrevia Luarte, L., Sáez, T., Vos, P.d., Kuipers, J. y Marijke, .F. (2018). Fetoplacental endothelial exosomes modulate high d-glucose-induced endothelial dysfunction. Placenta, 66, 26-35. | |
dc.identifier.issn | 1532-3102 | es |
dc.identifier.uri | https://hdl.handle.net/11441/85746 | |
dc.description.abstract | Gestational diabetes mellitus (GDM) is associated with fetoplacental endothelial dysfunction, which may be induced by hyperglycemia. We hypothesized that endothelial exosomes, which are extracellular nanovesicles affecting endothelial function, play a role in the high glucose (HG)-induced endothelial dysfunction.
METHODS:
Exosomes were isolated from HUVECs incubated with basal glucose (5.5 mmol/L; HUVEC- BG; exo-BG) and from HUVECs incubated with HG for 24 h (25 mmol/L; HUVEC-HG; exo-HG) in exosome-free medium. Exosomes were isolated and characterized by ultracentrifugation, sucrose gradient, electron microscopy, nanotracking analysis and Western blotting. HUVEC-BG and HUVEC-HG were exposed to exo-BG and exo-HG in two different concentrations: 5 μg and 1 μg exosome protein/mL. The exosomal effect on endothelial cell function was determined by wound healing assay, expression of endothelial nitric oxide synthase (eNOS), human cationic amino acid transporter type 1 (hCAT-1), vascular endothelial growth factor (VEGF) and intracellular adhesion molecule type 1 (ICAM-1) by Western blotting, qPCR or flow cytometry.
RESULTS:
HG increased the exosomal release from HUVECs, endothelial wound healing and expression of phosphorylated (P∼Ser1177)-eNOS, hCAT-1, VEGF and ICAM-1. Exo-HG also increased endothelial cell wound healing, P∼Ser1177-eNOS, hCAT-1 and ICAM-1 expression in HUVEC-BG. Exo-BG reverted the effect of HG on endothelial cell wound healing and hCAT-1 mRNA expression to normal values.
DISCUSSION:
Our results show that HG may induce endothelial dysfunction in HUVECs and that exosomes from HUVEC-HG mimicked some of the effects of HG. This study contributes to the unraveling of the mechanism by which hyperglycemia affects the fetoplacental vasculature in GDM. | es |
dc.description.sponsorship | 7th European Community Framework Program (grant agreement No. 295185 – EULAMDIMA) | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Placenta, 66, 26-35. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Endothelial dysfunction | es |
dc.subject | Exosomes | es |
dc.subject | Fetoplacental vasculature | es |
dc.subject | Gestational diabetes | es |
dc.subject | High glucose | es |
dc.title | Fetoplacental endothelial exosomes modulate high d-glucose-induced endothelial dysfunction | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología | es |
dc.relation.projectID | grant agreement No. 295185 – EULAMDIMA | es |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.placenta.2018.04.010 | es |
dc.identifier.doi | 10.1016/j.placenta.2018.04.010 | es |
idus.format.extent | 10 p. | es |
dc.journaltitle | Placenta | es |
dc.publication.volumen | 66 | es |
dc.publication.initialPage | 26 | es |
dc.publication.endPage | 35 | es |