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dc.creatorPapa Daouda, Mares
dc.creatorElkhalfi, Bouchraes
dc.creatorPérez Castiñeira, José Románes
dc.creatorSerrano Delgado, Aurelioes
dc.creatorSoukri, Abdelazizes
dc.date.accessioned2019-04-09T09:05:33Z
dc.date.available2019-04-09T09:05:33Z
dc.date.issued2017
dc.identifier.citationPapa Daouda, M., Elkhalfi, B., Pérez Castiñeira, J.R., Serrano Delgado, A. y Soukri, A. (2017). Cell stress by phosphate of two protozoa tetrahymena thermophila and tetrahymena pyriformis. Advances in Bioscience and Biotechnology, 8, 451-462.
dc.identifier.issn2156-8456 (impreso)es
dc.identifier.issn2156-8502 (electrónico)es
dc.identifier.urihttps://hdl.handle.net/11441/85380
dc.description.abstractPhosphorus is one of the bioelements most needed as a compound cell by living organisms. Phosphorus is involved in several pathologies: in human with bone and kidney diseases, in mammals with metabolism disorder (glucose, insulin···), in microorganisms whose phosphorus is involved in cell growth. Phosphorus has various forms including pyrophosphate, a by-product of multiple pathways of biosynthesis. Enzymes that hydrolyze pyrophosphate are called inorganic pyrophosphatases (PPases). Two major types of inorganic pyrophosphatases are distinguished: the soluble pyrophosphatases (sPPases) and the membrane pyrophosphatases (mPPases or H+/Na+-PPases). They play a key role in the control of intracellular inorganic pyrophosphate level and produce an important ions gradient (H+ or Na+) to the cells. In this work, we primarily focused on the physiological study in a phosphate-poor medium of two models Tetrahymena thermophile and Tetrahymena pyriformis, following the mobility, the growth and the morphology of cells. Secondly, we evaluated the enzymatic activity of soluble and membrane pyrophosphatases in both species grown in the same complex medium. A decrease of cell growth is correlated with unusual morphologies and different mobility in the stress medium. The measurement of soluble and membrane inorganic pyrophosphatases activities also shows a decrease which illustrates the lack of phosphate found in the stress medium. Deficiency of phosphate is a limiting factor for protozoan growth. These results indicate that Tetrahymena can be used as a model of cellular stress and consists of a target to study inorganic pyrophosphatases for a better understanding of phosphate cycle in higher organisms.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherScientific Research Publishinges
dc.relation.ispartofAdvances in Bioscience and Biotechnology, 8, 451-462.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectPhosphoruses
dc.subjectPyrophosphataseses
dc.subjectNBRIPes
dc.subjectTetrahymenaes
dc.titleCell stress by phosphate of two protozoa tetrahymena thermophila and tetrahymena pyriformises
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.publisherversionhttp://dx.doi.org/10.4236/abb.2017.812033es
dc.identifier.doi10.4236/abb.2017.812033es
idus.format.extent12 p.es
dc.journaltitleAdvances in Bioscience and Biotechnologyes
dc.publication.volumen8es
dc.publication.initialPage451es
dc.publication.endPage462es

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