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The sRNA NsiR4 is involved in nitrogen assimilation control in cyanobacteria by targeting glutamine synthetase inactivating factor IF7

 

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dc.creator Klähn, Stephan es
dc.creator Schaal, Christoph es
dc.creator Georg, Jen es
dc.creator Baumgartner, Desirée es
dc.creator Knippen, Gernot es
dc.creator Hagemann, Martin es
dc.creator Muro Pastor, Alicia María es
dc.creator Hess, Wolfgang R. es
dc.date.accessioned 2019-04-01T10:24:51Z
dc.date.available 2019-04-01T10:24:51Z
dc.date.issued 2015
dc.identifier.citation Klähn, S., Schaal, C., Georg, J., Baumgartner, D., Knippen, G., Hagemann, M.,...,Hess, W.R. (2015). The sRNA NsiR4 is involved in nitrogen assimilation control in cyanobacteria by targeting glutamine synthetase inactivating factor IF7. Proceedings of the National Academy of Sciences, 112 (45), E6243-E6252.
dc.identifier.issn 0027-8424 (impreso) es
dc.identifier.issn 1091-6490 (electrónico) es
dc.identifier.uri https://hdl.handle.net/11441/84987
dc.description.abstract Glutamine synthetase (GS), a key enzyme in biological nitrogen assimilation, is regulated in multiple ways in response to varying nitrogen sources and levels. Here we show a small regulatory RNA, NsiR4 (nitrogen stress-induced RNA 4), which plays an important role in the regulation of GS in cyanobacteria. NsiR4 expression in the unicellular Synechocystis sp. PCC 6803 and in the filamentous, nitrogen-fixing Anabaena sp. PCC 7120 is stimulated through nitrogen limitation via NtcA, the global transcriptional regulator of genes involved in nitrogen metabolism. NsiR4 is widely conserved throughout the cyanobacterial phylum, suggesting a conserved function. In silico target prediction, transcriptome profiling on pulse overexpression, and site-directed mutagenesis experiments using a heterologous reporter system showed that NsiR4 interacts with the 5′UTR of gifA mRNA, which encodes glutamine synthetase inactivating factor (IF)7. In Synechocystis, we observed an inverse relationship between the levels of NsiR4 and the accumulation of IF7 in vivo. This NsiR4-dependent modulation of gifA (IF7) mRNA accumulation influenced the glutamine pool and thus NH+4 assimilation via GS. As a second target, we identified ssr1528, a hitherto uncharacterized nitrogen-regulated gene. Competition experiments between WT and an ΔnsiR4 KO mutant showed that the lack of NsiR4 led to decreased acclimation capabilities of Synechocystis toward oscillating nitrogen levels. These results suggest a role for NsiR4 in the regulation of nitrogen metabolism in cyanobacteria, especially for the adaptation to rapid changes in available nitrogen sources and concentrations. NsiR4 is, to our knowledge, the first identified bacterial sRNA regulating the primary assimilation of a macronutrient. es
dc.description.sponsorship Federal Ministry of Education and Research 0316183 es
dc.description.sponsorship Ministerio de Economía y Competitividad BFU2013‐4828‐C2‐1­‐P es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher National Academy of Sciences es
dc.relation.ispartof Proceedings of the National Academy of Sciences, 112 (45), E6243-E6252.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject Regulatory RNA es
dc.subject Synechocystis es
dc.subject Nitrogen assimilation es
dc.subject Glutamine synthetase inactivating factors es
dc.subject NtcA es
dc.title The sRNA NsiR4 is involved in nitrogen assimilation control in cyanobacteria by targeting glutamine synthetase inactivating factor IF7 es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/acceptedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular es
dc.relation.projectID 0316183 es
dc.relation.projectID BFU2013‐4828‐C2‐1­‐P es
dc.relation.publisherversion https://doi.org/10.1073/pnas.1508412112 es
dc.identifier.doi 10.1073/pnas.1508412112 es
idus.format.extent 10 p. es
dc.journaltitle Proceedings of the National Academy of Sciences es
dc.publication.volumen 112 es
dc.publication.issue 45 es
dc.publication.initialPage E6243 es
dc.publication.endPage E6252 es
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