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Arabidopsis S-Sulfocysteine Synthase Activity Is Essential for Chloroplast Function and Long-Day Light-Dependent Redox Control

 

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dc.creator Bermúdez Alcántara, María Ángeles es
dc.creator Páez Ochoa, María Ángeles es
dc.creator Gotor Martínez, Cecilia es
dc.creator Romero González, Luis Carlos es
dc.date.accessioned 2019-03-28T11:42:26Z
dc.date.available 2019-03-28T11:42:26Z
dc.date.issued 2010
dc.identifier.citation Bermúdez Alcántara, M.Á., Páez Ochoa, M.Á., Gotor Martínez, C. y Romero González, L.C. (2010). Arabidopsis S-Sulfocysteine Synthase Activity Is Essential for Chloroplast Function and Long-Day Light-Dependent Redox Control. Plant Cell, 22, 403-416.
dc.identifier.issn 1040-4651 (impreso) es
dc.identifier.issn 1532-298X (electrónico) es
dc.identifier.uri https://hdl.handle.net/11441/84858
dc.description.abstract In bacteria, the biosynthesis of Cys is accomplished by two enzymes that are encoded by the cysK and cysM genes. CysM is also able to use thiosulfate as a substrate to produce S-sulfocysteine. In plant cells, the biosynthesis of Cys occurs in the cytosol, mitochondria, and chloroplasts. Chloroplasts contain two O-acetylserine(thiol)lyase homologs, which are encoded by the OAS-B and CS26 genes in Arabidopsis thaliana. An in vitro enzymatic analysis of the recombinant CS26 protein demonstrated that this isoform possesses S-sulfocysteine synthase activity and lacks O-acetylserine(thiol)lyase activity. In vivo functional analysis of this enzyme in knockout mutants demonstrated that mutation of CS26 suppressed the S-sulfocysteine synthase activity that was detected in the wild type; furthermore, the cs26 mutants exhibited a reduction in size and showed paleness, but penetrance of the growth phenotype depended on the light regime. The cs26 mutant plants also had reductions in chlorophyll content and photosynthetic activity (neither of which were observed in oas-b mutants) as well as elevated glutathione levels. However, cs26 leaves were not able to properly detoxify reactive oxygen species, which accumulated to high levels under long-day growth conditions. The transcriptional profile of the cs26 mutant revealed that the mutation had a pleiotropic effect on many cellular and metabolic processes. Our findings reveal that S-sulfocysteine and the activity of S-sulfocysteine synthase play important roles in chloroplast function and are essential for light-dependent redox regulation within the chloroplast. es
dc.description.sponsorship Ministerio de Ciencia e Innovación BIO2007-62770 CSD2007-0005 es
dc.description.sponsorship Junta de Andalucía P06-CVI-01737 BIO-273 es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher American Society of Plant Biologists es
dc.relation.ispartof Plant Cell, 22, 403-416.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.title Arabidopsis S-Sulfocysteine Synthase Activity Is Essential for Chloroplast Function and Long-Day Light-Dependent Redox Control es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/acceptedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular es
dc.relation.projectID BIO2007-62770 es
dc.relation.projectID CSD2007-0005 es
dc.relation.projectID P06-CVI-01737 es
dc.relation.projectID BIO-273 es
dc.relation.publisherversion https://doi.org/10.1105/tpc.109.071985 es
dc.identifier.doi 10.1105/tpc.109.071985 es
idus.format.extent 15 p. es
dc.journaltitle Plant Cell es
dc.publication.volumen 22 es
dc.publication.initialPage 403 es
dc.publication.endPage 416 es
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