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Mutation in the pssZ Gene Negatively Impacts Exopolysaccharide Synthesis, Surface Properties, and Symbiosis of Rhizobium leguminosarum bv. trifolii with Clover

 

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dc.creator Vinardell González, José María es
dc.creator Lipa, Paulina es
dc.creator Kopcińska, Joanna es
dc.creator Zdybicka Barabas, Agnieszka es
dc.creator Janczarek, Monika es
dc.date.accessioned 2018-08-29T11:18:10Z
dc.date.available 2018-08-29T11:18:10Z
dc.date.issued 2018
dc.identifier.citation Vinardell González, J.M., Lipa, P., Kopcińska, J., Zdybicka Barabas, A. y Janczarek, M. (2018). Mutation in the pssZ Gene Negatively Impacts Exopolysaccharide Synthesis, Surface Properties, and Symbiosis of Rhizobium leguminosarum bv. trifolii with Clover. Genes, 9 (7), 1-27.
dc.identifier.issn 2073-4425 es
dc.identifier.uri https://hdl.handle.net/11441/78283
dc.description.abstract Rhizobium leguminosarum bv. trifolii is a soil bacterium capable of establishing a nitrogen-fixing symbiosis with clover plants (Trifolium spp.). This bacterium secretes large amounts of acidic exopolysaccharide (EPS), which plays an essential role in the symbiotic interaction with the host plant. This polymer is biosynthesized by a multi-enzymatic complex located in the bacterial inner membrane, whose components are encoded by a large chromosomal gene cluster, called Pss-I. In this study, we characterize R. leguminosarum bv. trifolii strain Rt297 that harbors a Tn5 transposon insertion located in the pssZ gene from the Pss-I region. This gene codes for a protein that shares high identity with bacterial serine/threonine protein phosphatases. We demonstrated that the pssZ mutation causes pleiotropic effects in rhizobial cells. Strain Rt297 exhibited several physiological and symbiotic defects, such as lack of EPS production, reduced growth kinetics and motility, altered cell-surface properties, and failure to infect the host plant. These data indicate that the protein encoded by the pssZ gene is indispensable for EPS synthesis, but also required for proper functioning of R. leguminosarum bv. trifolii cells. es
dc.description.sponsorship Polish National Science Centre grant no. DEC-2012/07/B/NZ1/00099 es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher MDPI es
dc.relation.ispartof Genes, 9 (7), 1-27.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject Rhizobium leguminosarum es
dc.subject serine/threonine protein phosphatase es
dc.subject exopolysaccharide synthesis es
dc.subject cell-surface properties es
dc.subject symbiosis es
dc.subject clover es
dc.title Mutation in the pssZ Gene Negatively Impacts Exopolysaccharide Synthesis, Surface Properties, and Symbiosis of Rhizobium leguminosarum bv. trifolii with Clover es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Microbiología es
dc.relation.projectID PoliDEC-2012/07/B/NZ1/00099 es
dc.relation.publisherversion http://dx.doi.org/10.3390/genes9070369 es
dc.identifier.doi 10.3390/genes9070369 es
idus.format.extent 27 p. es
dc.journaltitle Genes es
dc.publication.volumen 9 es
dc.publication.issue 7 es
dc.publication.initialPage 1 es
dc.publication.endPage 27 es
dc.contributor.funder National Science Center. Poland
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